Introduction
The MagPure Universal RNA Kit (IVD3020) is designed for automated purification of total RNA from tissues, cultured cells and urine-derived cellular samples using magnetic bead purification technology. Magnetic particle-based extraction enables efficient RNA recovery while supporting automated workflows for high-throughput laboratories.
The purification chemistry allows RNA molecules to bind selectively to magnetic particles under optimized buffer conditions. Magnetic separation, combined with DNase I treatment, supports removal of proteins, genomic DNA and other contaminants during washing steps.
Within the Magen RNA extraction systems, the MagPure Universal RNA Kit represents the magnetic bead-based RNA extraction platform for automated total RNA purification from routine biological samples, including tissues, cultured cells and urine-derived cell pellets.
Details
Specifications
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Features
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Specifications
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Main Functions
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Isolation total RNA from tissue, cell, urine
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Applications
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RT-PCR, cDNA synthesis, second generation sequencing
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Purification method
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Polydisperse magnetic beads
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Purification technology
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Magnetic beads technology
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Process method
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Manual or automatic
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Adaptive instrument
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Nucleic acid extractor, pipetting workstation
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Sample type
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Tissues, cells, lymphocytes and other clinical sample
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Sample amount
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Cells grown in suspension:3~5 x 106
Animal tissue: 10~20mg
Plant tissue: ≤100mg
Urine: ≤10ml
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Engineering Characteristics
Magnetic bead RNA purification
RNA molecules selectively bind to magnetic particles, enabling efficient purification through magnetic separation.
Automation-ready extraction workflow
The system supports integration with automated nucleic acid extraction instruments and multi-channel purification platforms.
Broad sample compatibility
The extraction chemistry supports purification of RNA from tissues, cultured cells and other biological samples.
Stable purification performance
Magnetic bead extraction reduces manual variability and provides consistent RNA recovery across multiple samples.
Technical Validation
Validation experiments demonstrated stable RNA purification across multiple biological samples processed using magnetic bead extraction workflows.
Purified RNA showed purity ratios consistent with high-quality RNA suitable for RT-PCR and transcriptomic analysis workflows.
Comparative evaluation with commonly used RNA purification systems demonstrated comparable RNA purity and yield, supporting reliable performance in automated RNA extraction laboratories.
Typical Applications
• Gene expression analysis
• RT-PCR workflows
• RNA sequencing sample preparation
• Transcriptomics studies
• High-throughput RNA purification laboratories
Related Products
• HiPure Total RNA Plus Kit (R4111) – column-based purification of high-purity RNA with genomic DNA removal
• HiPure Universal RNA Kit (R4130) – purification of RNA including small RNA fractions
• MagPure Blood RNA Kit (R6611) – automated RNA purification from blood samples
• HiPure Universal miRNA Kit (R4310) – enrichment of small RNA molecules for gene regulation studies
Kit Contents
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Contents
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IVD3020
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Purification Times
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200 Preps
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MagPure RNA Particles
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7 ml
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DNase I
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4 x 600 µl
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DNase Buffer
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80 ml
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RTL Lysis Buffer
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150 ml
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Buffer MCB*
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75 ml
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Buffer MW1*
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110 ml
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Buffer MW2*
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50 ml
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RNase Free Water
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60 ml
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Cat.No
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Reagent
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IVD3020-F-96
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DNase Buffer
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60 ml
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DNase I
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2 x 600 μl
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RTL Lysis Buffer
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80 ml
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Buffer MCB
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18 ml
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96-Tip
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1
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Sample plate (DW Plate)
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500μl Buffer MCB
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1
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Wash 1 Plate (DW Plate)
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700μl Buffer MW1
30μl MagPure RNA Partilces
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1
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DNase Plate
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Empty
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Wash 2 Plate (DW Plate)
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700μl Buffer MW1
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1
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Wash 3 Plate (DW Plate)
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900μl Buffer MW2
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1
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Elution plate (DW Plate)
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80μl RNase Free Water
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1
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Cat.No
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Reagent
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IVD3020-TL-06
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Purification times
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96 Preps
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DNase I
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2 x 600 μl
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DNase Buffer
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60 ml
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RTL Lysis Buffer
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60 ml
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Buffer MCB
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40 ml
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96-Tip
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12 PCS
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2.0ml V-bottom plate
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Row 1/7：500μl Buffer MCB
Row 2/8：500μl Buffer MW1
Row 3/9：empty
Row 4/10：30μl Magpure RNA Particles
500μl Buffer MW2
Row 5/11：900μl Buffer MW2
Row 6/12：80μl RNase Free Water
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6
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Storage and Stability
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at roomtemperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
