MagPure Universal RNA Kit

The MagPure Universal RNA Kit (IVD3020) is designed for automated purification of total RNA from tissues, cultured cells and urine-derived cellular samples using magnetic bead purification technology. Magnetic particle-based extraction enables efficient RNA recovery while supporting automated workflows for high-throughput laboratories.

The purification chemistry allows RNA molecules to bind selectively to magnetic particles under optimized buffer conditions. Magnetic separation, combined with DNase I treatment, supports removal of proteins, genomic DNA and other contaminants during washing steps.

Within the Magen RNA extraction systems, the MagPure Universal RNA Kit represents the magnetic bead-based RNA extraction platform for automated total RNA purification from routine biological samples, including tissues, cultured cells and urine-derived cell pellets.

Workflow

Workflow Overview

The MagPure Universal RNA Kit with DNase I uses a magnetic bead–based workflow for purification of total RNA from tissue, cultured cells, plant material, urine cell pellets and other compatible clinical or biological samples. Following sample disruption and lysis in RTL Lysis Buffer, RNA binding conditions are established with Buffer MCB and MagPure RNA Particles. RNA is captured on the magnetic particles, separated by magnetic handling and purified through washing, DNase I treatment, re-binding, additional washing, drying and elution in RNase-free water. The workflow combines silica-based RNA binding chemistry with magnetic-particle handling for manual or automated RNA extraction.

Sample Handling Logic

This workflow is designed for sample types where magnetic handling and DNA background control are important. Soft tissue, cultured cells and plant samples mainly differ in the disruption and clarification stage, while urine samples are first centrifuged to collect cellular material before lysis. Once the lysate or clarified supernatant enters the magnetic binding step, the downstream workflow follows a consistent logic: RNA binding to magnetic particles, first wash, DNase I treatment, restoration of binding conditions with Buffer MCB, final washes, drying and elution. The workflow can also accommodate selected Trizol / MagZol lysates depending on the sample preparation route.

Time and Workflow Characteristics

Under typical manual operation, the workflow is usually completed within about 85–105 minutes, depending mainly on sample pretreatment, lysate viscosity, DNase incubation and magnetic drying time. For automated operation on 32 / 48-channel or 96-channel extraction systems, the workflow is designed with a programmed pause to add Buffer MCB after DNase treatment, allowing the DNA digestion step to be integrated into the automated magnetic workflow. This route is suitable for laboratories that need reproducible magnetic RNA extraction with built-in DNase support and compatibility with manual or automated processing. For detailed step-by-step conditions, workflow guidance and estimated processing times, please refer to the Workflow Note in the Download section.

Specifications

Engineering Characteristics

Magnetic bead RNA purification

RNA molecules selectively bind to magnetic particles, enabling efficient purification through magnetic separation.

Automation-ready extraction workflow

The system supports integration with automated nucleic acid extraction instruments and multi-channel purification platforms.

Broad sample compatibility

The extraction chemistry supports purification of RNA from tissues, cultured cells and other biological samples.

Stable purification performance

Magnetic bead extraction reduces manual variability and provides consistent RNA recovery across multiple samples.

Technical Validation

Validation experiments demonstrated stable RNA purification across multiple biological samples processed using magnetic bead extraction workflows.

Purified RNA showed purity ratios consistent with high-quality RNA suitable for RT-PCR and transcriptomic analysis workflows.

Comparative evaluation with commonly used RNA purification systems demonstrated comparable RNA purity and yield, supporting reliable performance in automated RNA extraction laboratories.

Typical Applications

Related Products

• HiPure Total RNA Plus Kit (R4111) – column-based purification of high-purity RNA with genomic DNA removal

• HiPure Universal RNA Kit (R4130) – purification of RNA including small RNA fractions

• MagPure Blood RNA Kit (R6611) – automated RNA purification from blood samples

• HiPure Universal miRNA Kit (R4310) – enrichment of small RNA molecules for gene regulation studies

Kit Contents

Storage and Stability

MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at roomtemperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

For a broader view of Magen tissue and cell RNA extraction routes, the following resources may help place this product within the complete workflow system.