The MagPure Total RNA Kit uses magnetic particle purification combined with Proteinase K digestion for RNA extraction from cells and tissue samples with high protein content or complex lysates. Proteinase K digestion helps degrade protein components and reduce lysate complexity, improving RNA binding efficiency to magnetic particles and supporting stable RNA purification from protein-rich samples.

After sample lysis, RNA binds to magnetic particles under optimized binding conditions while Proteinase K digestion reduces residual protein interference. The captured RNA is purified through a series of washing steps and finally eluted in RNase-free water. The workflow is compatible with both automated nucleic acid extraction platforms and routine laboratory RNA purification.

Within the Magen RNA extraction systems, automated universal RNA purification workflows are supported by the MagPure Universal RNA Kit (IVD3020), while column-based RNA purification can be performed using the HiPure Total RNA Plus Kit (R4111).

Specifications

Principle

The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.

Kit Contents

Storage and Stability

MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNase I shouldbe stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles and Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for atleast 18 months under these conditions.

Choosing the appropriate RNA extraction kits depends on sample type, RNA species and workflow requirements.

For detailed guidance across tissue and cell RNA extraction workflows, refer to the Tissue/Cell RNA Kits Selection Guide.