Magnetic Bead Blood RNA Extraction Kit for Frozen Samples

Introduction

The MagPure Blood RNA Kit II integrates MagZol reagent lysis with magnetic particle purification to enable RNA extraction directly from fresh or frozen blood samples. The workflow eliminates the need for red blood cell lysis and supports RNA purification from blood samples stored under frozen or transport conditions.

This system is particularly suitable for laboratories processing biobank samples or frozen blood samples where conventional leukocyte enrichment workflows are not applicable.

For routine automated blood RNA purification laboratories may use the MagPure Blood RNA Kit (R6611).

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from 1ml anticoagulant blood (fresh or frozen blood/cryopreservation blood), lymphocytes, buffy coat, bone marrow, cultured cells, etc using Magzol reagents.
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells
Sample amount	1ml whole blood (fresh or frozen blood)

Principles

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested by lysis buffer and protease, and RNA/DNA is released into the lysis buffer. Add binding solution and magnetic particles to adsorb RNA/DNA, while proteins are not adsorbed and removed. The particles adsorbed with DNA/RNA are washed with washing buffer to remove proteins and other impurities, then washed with ethanol to remove salt, and finally digested with DNase to remove DNA. RNA is recovered by adding binding solution, and finally the RNA is eluted with low salt buffer. The eluted RNA can be directly used for experiments such as RT-PCR, NGS and virus detection.

Kit Contents

Contents	R661301	R661302	R661303
Purification Times	48 Preps	96 Preps	480 preps
DNase I	600 μl	2 x 600 μl	10 x 600 µl
DNase Buffer C	20 ml	30 ml	150 ml
MagPure Particles N	2.5 ml	5.0 ml	28 ml
MagZol 3BD	65 ml	140 ml	3 x 200 ml
Buffer GW1*	13 ml	44 ml	110 ml
Buffer BCP2	10 ml	15 ml	80 ml
Buffer MW2*	20 ml	50 ml	2 x 100 ml
RNase Free Water	10 ml	20 ml	60 ml

Kit Contents: Precast kit

Cat.No	Reagent	R6613-TL-06
DNase I		2 x 600 μl
Magzol 3BD		130 ml
Buffer BCP2		15 ml
AS Tip		12 PCS
2.0ml V-bottom plate	Row 1/7：350μl Isopropanol 15μl MagPure Particles N Row 2/8：350μl Isopropanol 15μl MagPure Particles N Row 3/9: 500μl DNase Buffer C Row 4/10: 500μl Buffer GW1 Row 5/11: 500μl Buffer MW2 Row 6/12：70μl RNase Free Water	6 Plates

Storage and Stability

DNase I should be shipped with ice pack or dry ice and stored at -20°C upon arrival. MagPure Particles N, MagZol 3BD and Buffer BCP2 should be stored at 2–8°C upon arrival. However, short-term storage at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

Choosing the appropriate RNA extraction system depends on sample type, RNA species and workflow requirements.

For detailed selection guidance across blood and liquid RNA workflows, refer to the Blood RNA Kits Selection Guide.

CAT NO	PRODUCT NAME	SIZE	PRICE
R661301	MagPure Blood RNA Kit Ⅱ	48 preps	$286.00
R661302	MagPure Blood RNA Kit Ⅱ	96 preps	$525.00
R661303	MagPure Blood RNA Kit Ⅱ	480 preps	$2,100.00
R6613-TL-06	MagPure Blood RNA Precast Kit II	96 preps	$588.00

MagPure Blood RNA Kit Ⅱ

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Details

Purchase Guide

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