Introduction
The MagPure Universal RNA Kit II integrates MagZol reagent lysis with magnetic particle purification to enable RNA extraction from heterogeneous or difficult biological samples. The MagZol lysis chemistry improves sample disruption for tissues, plant samples, microbial samples and other complex materials.
After MagZol-based lysis, RNA molecules are captured by magnetic particles and purified through sequential washing steps to remove proteins and organic contaminants. The workflow supports both manual and automated extraction systems and is suitable for laboratories processing diverse sample types.
For routine automated RNA purification workflows laboratories may use the MagPure Universal RNA Kit (IVD3020), while column-based RNA extraction is supported by the HiPure Total RNA Plus Kit (R4111).
Details
Workflow
Workflow Overview
The MagPure Universal RNA Kit II uses the same MagZol lysis and phase-separation logic as the column-based route, but connects the recovered aqueous RNA phase to magnetic particle purification. Samples are lysed in MagZol Reagent, and Buffer BCP or chloroform is used to support phase separation. After centrifugation, the RNA-containing aqueous phase is combined with isopropanol and MagPure Particles N, allowing RNA to bind to the magnetic particles. The bound RNA is then purified through magnetic separation, washing, drying and elution in RNase-free water.
Sample Handling Logic
This workflow is designed for broad universal RNA extraction from cultured cells, animal tissues, blood or bone marrow cell pellets, blood vessels, bone, plant material, fungi, bacteria and related sample types. The shared front-end logic is MagZol-based lysis and phase separation; the downstream difference is the magnetic recovery format. Manual operation can be performed with a magnetic rack, while the same binding, washing and elution logic can also be adapted to compatible automated extraction systems. This makes the workflow suitable when laboratories require stronger MagZol front-end processing together with magnetic handling or batch processing.
Time and Workflow Characteristics
Under typical manual operation, the overall workflow is usually completed within about 70–90 minutes, depending on sample pretreatment, phase separation and magnetic drying time. For automated processing, the magnetic purification program is shorter once the phase-separated aqueous sample has been prepared and loaded, with the instrument purification stage typically completed in about 30 minutes under the recommended program. This route provides a magnetic alternative to the column workflow while retaining the same MagZol phase-separation front end. For detailed step-by-step conditions, workflow guidance and estimated processing times, please refer to the Workflow Note in the Download section.
Kit Contents
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Contents
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R662301
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R662302
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R662303
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|
Purification Times
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48 Preps
|
96 Preps
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480 Preps
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|
MagPure Particles N
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1.7 ml
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3.5 ml
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17 ml
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MagZol Reagent
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60 ml
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120 ml
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2 x 250 ml
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Buffer BCP
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10 ml
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15 ml
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60 ml
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Buffer MW1*
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22 ml
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44 ml
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2 x 110 ml
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Buffer MW2*
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20 ml
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50 ml
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3 x 50 ml
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RNase Free Water
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15 ml
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30 ml
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150 ml
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Storage and Stability
MagPure Particles N, MagZol Reagent and Buffer BCP should be stored at 2–8°C upon arrival. However, short-term storage at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
