Introduction

The MagPure Universal RNA Kit II integrates MagZol reagent lysis with magnetic particle purification to enable RNA extraction from heterogeneous or difficult biological samples. The MagZol lysis chemistry improves sample disruption for tissues, plant samples, microbial samples and other complex materials.

After MagZol-based lysis, RNA molecules are captured by magnetic particles and purified through sequential washing steps to remove proteins and organic contaminants. The workflow supports both manual and automated extraction systems and is suitable for laboratories processing diverse sample types.

For routine automated RNA purification workflows laboratories may use the MagPure Universal RNA Kit (IVD3020), while column-based RNA extraction is supported by the HiPure Total RNA Plus Kit (R4111).

Details

Principle

The product is based on superparamagnetic particles purification technology. Samples are lysed and digested by Magzol Reagent, and DNA/RNA is released into the lysis buffer. Genomic DNA and protein impurities are removed after adding Buffer BCP (or chloroform). RNA is absorb on magnetic particles with binding buffer. Protein and other impurities are removed by wash buffer, and salts are remove by ethanol condition. Purified is eluted in low salt buffers ( RNase Free Water).

Kit Contents

Contents	R662301	R662302	R662303
Purification Times	48 Preps	96 Preps	480 Preps
MagPure Particles N	1.7 ml	3.5 ml	17 ml
MagZol Reagent	60 ml	120 ml	2 x 250 ml
Buffer BCP	10 ml	15 ml	60 ml
Buffer MW1*	22 ml	44 ml	2 x 110 ml
Buffer MW2*	20 ml	50 ml	3 x 50 ml
RNase Free Water	15 ml	30 ml	150 ml

Storage and Stability

MagPure Particles N, MagZol Reagent and Buffer BCP should be stored at 2–8°C upon arrival. However, short-term storage at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

Choosing the appropriate RNA extraction kits depends on sample type, RNA species and workflow requirements.

For detailed guidance across tissue and cell RNA extraction workflows, refer to the Tissue/Cell RNA Kits Selection Guide.

Articles

Download

R6623 MagPure Universal RNA Kit II (Magzol).pdf Preview Down

CAT NO	PRODUCT NAME	SIZE	PRICE
R662301	MagPure Universal RNA Kit II	48 preps	$244.00
R662302	MagPure Universal RNA Kit II	96 preps	$418.00
R662303	MagPure Universal RNA Kit II	480 preps	$1,880.00

MagPure Universal RNA Kit II

Introduction

Details

Principle

Purchase Guide

Articles

Download

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