MagPure Universal RNA Kit B

The MagPure Universal RNA Kit B is optimized for automated RNA purification from low-input biological samples using magnetic bead technology. The workflow supports continuous automated extraction without pause steps and is compatible with automated liquid handling platforms used in clinical or high-throughput laboratories.

RNA molecules are captured by magnetic silica particles and purified through sequential washing steps to remove proteins and salts. The system supports RNA purification from cells, tissues and low-input biological samples where automated workflows are preferred.

For standard automated RNA purification workflows laboratories may use the MagPure Universal RNA Kit (IVD3020), while column-based RNA purification workflows are supported by the HiPure Total RNA Plus Kit (R4111).

Workflow

Workflow Overview

The MagPure Universal RNA Kit B with DNase I uses a magnetic bead–based workflow for rapid total RNA extraction from low-RNA-yield samples, including small tissue inputs, cultured cells, bone marrow, fresh blood and related clinical samples. Following lysis in Buffer RLC, RNA binding conditions are established with Buffer MCB and MagPure Particles N. RNA is captured on the magnetic particles, purified through washing, DNase I treatment, additional washing, drying and elution in RNase-free water. The workflow follows the same magnetic binding principle as the standard automated RNA route, but is configured for low-input samples and automated processing without a mid-run pause.

Sample Handling Logic

This workflow is designed for sample types where RNA input may be limited and stable magnetic recovery is important. Small tissue samples, cultured cells, plant material and blood or bone marrow leukocyte pellets require appropriate front-end lysis and viscosity control before entering the magnetic binding step. Once the lysate or clarified supernatant is loaded, the downstream process follows a consistent magnetic format: RNA binding to particles, first wash, DNase treatment, final washes and elution. For difficult plant samples, alternative lysis buffers may be selected when polysaccharides, polyphenols or starch-rich matrices interfere with standard RLC-based lysis.

Time and Workflow Characteristics

Under typical manual operation, the workflow is usually completed within about 65–100 minutes, depending mainly on sample preparation, DNase incubation and magnetic drying time. For automated 32 / 48-channel or 96-channel extraction systems, the workflow is designed as an automated-without-pause format, with the instrument purification stage typically completed in about 35 minutes after plate setup. This route is suitable for laboratories that require low-input RNA recovery, DNase-supported purification and streamlined magnetic automation without manual interruption during the run. For detailed step-by-step conditions, workflow guidance and estimated processing times, please refer to the Workflow Note in the Download section.

Specifications

Kit Contents

Storage and Stability

DNase I should be shipped with ice pack and stored at -20°C after arrival. MagPure Particles N should be stored at 2–8°C for long time storage. The remaining kit components can be stored at room temperature(15–25°C) for up to18 months under these conditions.

For a broader view of Magen tissue and cell RNA extraction routes, the following resources may help place this product within the complete workflow system.