MagBind Particles

MagBind Particles are carboxyl-coated magnetic beads designed for nucleic acid purification workflows based on PEG/NaCl binding chemistry. Unlike silica-based magnetic beads, DNA adsorption on MagBind Particles occurs through crowding-induced precipitation under controlled polyethylene glycol conditions, enabling efficient recovery of nucleic acids across a broad fragment size range.

This binding mechanism supports applications where controlled DNA recovery or size selection is required, including cleanup of enzymatic reactions and fragment enrichment workflows. The nonporous and monodisperse particle structure contributes to reproducible magnetic response and consistent separation behavior across different sample conditions.

MagBind Particles are commonly integrated into workflows requiring flexible nucleic acid handling, including FFPE DNA/RNA purification systems and PEG-based cleanup processes, where stable recovery and predictable binding behavior are critical for downstream molecular analysis.

Specifications

Principle

Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.

Alcohol mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.

After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.

Ordering information

Related Bead Selection

• MagPure Particles N (C1411)

  → Recommended for nucleic acid extraction from low-input or viral samples

• MagPure Particles F (C1414)

  → Suitable for upstream cfDNA extraction prior to clean-up workflows

• MagPure Particles (C1410)

  → General-purpose DNA/RNA extraction workflows

For full comparison across all magnetic bead systems, refer to the Magnetic Bead Selection Guide.