MagPure Particles F

MagPure Particles F are developed for high-efficiency nucleic acid purification from large-volume genomic DNA/RNA, especially circulating DNA (cfDNA) samples. The system is optimized to support consistent recovery of fragmented DNA from plasma inputs where maximizing yield is critical.

The magnetic bead surface and binding conditions are designed to maintain stable adsorption across varying sample volumes, enabling reliable performance in workflows involving increased plasma input or low-abundance nucleic acids.

Compared with small-volume extraction systems, MagPure Particles F provide improved recovery efficiency in larger input workflows. For applications involving limited sample input or smaller plasma volumes, MagPure Particles G may be considered as an alternative solution.

Specifications

Principle

Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.

Alcohol mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.

After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.

Technical Validation

MagPure Particles F were evaluated as monodisperse silica magnetic beads for low-input DNA recovery, short-fragment DNA recovery and plasma nucleic acid extraction workflows. In small-volume recovery testing using 20 ng of 50 bp DNA marker, MagPure Particles F achieved recovery rates above 90%, supporting efficient recovery of trace short DNA fragments.

Large-volume low-input recovery was tested using 100 ng of 50 bp DNA marker in a 4 mL sample volume. MagPure Particles F showed actual elution recovery above 80% and obtained recovery above 75% under the tested conditions. The bead format also reduced elution-volume loss carried by the beads, with measured elution loss controlled at approximately 8 µL in the tested workflow.

Short-fragment recovery was further tested by adding 50 bp DNA marker into porcine plasma. MagPure Particles F recovered short DNA fragments with recovery rates of approximately 91–96%. The purified DNA showed A260/280 values around 1.81–1.86 and A260/230 values around 1.81–1.83 under the tested conditions.

Human plasma extraction was evaluated in both small-volume and large-volume workflows. In 300 µL and 4 mL plasma samples, MagPure Particles F produced measurable Qubit-based DNA recovery and showed stable performance in low-input plasma nucleic acid extraction workflows. These results support its use in plasma, cfDNA and other low-input nucleic acid extraction systems where short-fragment recovery and low elution-volume loss are important.

Ordering information

Related Bead Selection

• MagPure Particles G (C1412)

  → Suitable for small-volume cfDNA and low-input workflows

• MagPure Particles N (C1411)

  → Recommended for viral nucleic acid and low-abundance samples

• MagBind Particles (C1413)

  → Designed for FFPE DNA/RNA isolation and NGS library preparation

For full comparison across all magnetic bead systems, refer to the Magnetic Bead Selection Guide.