Introduction

HiPure FastFilter Plasmid Maxi Kit extendsthe standard plasmid column workflow into larger-input preparation whilepreserving the same FastFilter clarification logic used in P1013. It isdesigned for users who need higher total DNA yield and more efficient lysatehandling from large bacterial culture volumes.

Within the standard plasmid system, P1014functions as the upper-end manual support product. Laboratories working atroutine small scale should stay with P1001 or P1002, while users requiringlow-endotoxin large-scale plasmid DNA should move to the EF core product, P1156B. For even larger low-endotoxin production, HiPure Plasmid EF Mega Kit(P1116) provides the dedicatedmega-scale route.

Details

Specifications

Features	Specifications
Main Functions	Isolation up to 1mg plasmid DNA from 200ml bacterial culture
Applications	Enzyme digestion, sequencing, PCR and labeling, etc.
Purification method	Maxi spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid, plasmid less than 30KB
Sample amount	100-200ml
Yield	0.4-1mg
Elution volume	≥500μl
Time per run	≤60 minutes
Liquid carrying volume per column	20ml
Binding yield of column	1mg

Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast - it takes only 15-60 minutes to complete the isolation

Kit Contents

Contents	P101402	P101403
Purification Times	10 Preps	50 Preps
RNase A	20 mg	40 mg
Buffer P1	140 ml	2 x 350 ml
Buffer P2	140 ml	2 x 350 ml
Buffer LEN3	70 ml	350 ml
Buffer GBT	120 ml	550 ml
Buffer PW1	60 ml	300 ml
Buffer PW2*	50 ml	4 x 100 ml
Elution Buffer	20 ml	120 ml
HiPure DNA Maxi Columns III	10	50
Lysate Clear Maxi Syringe	10	50
50 ml Collection Tubes	20	100

Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.

Purchase Guide

For guidance on selecting between standard and low-endotoxin plasmid workflows based on copy number, culture input and processing format, please refer to the Plasmid DNA Kits Purchase Guide.

Articles

Engineering endogenous ABC transporter with improving ATP supply and membrane flexibility enhances the secretion of β-carotene in Saccharomyces cerevisiae

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P1014 HiPure FastFilter Plasmid Maxi Kit 2022.pdf Preview Down

CAT NO	PRODUCT NAME	SIZE	PRICE
P101402	HiPure Fastfilter Plasmid Maxi Kit	10 preps	$199.00
P101403	HiPure Fastfilter Plasmid Maxi Kit	50 preps	$746.00

HiPure Fastfilter Plasmid Maxi Kit

Introduction

Details

Principle

Purchase Guide

Articles

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