Introduction

HiPure Plasmid Plus 96 Kit is the standard96-well support product within the Magen plasmid portfolio. It transfers thestandard HiPure column chemistry into a plate-based format for laboratoriesthat need parallel plasmid preparation rather than one-by-one manual minipreps.

This product is best positioned forscreening-scale workflows and batch processing. For single-sample routinemanual preparation, HiPure Plasmid Mini Kit (P1001) remains the standard core reference. Forlow-endotoxin plate-based preparation, HiPure Plasmid EF 96 Kit (P1157) is thealigned support product within the EF branch. Laboratories preferring magneticseparation rather than vacuum manifold handling may refer to MagPure PlasmidMini Kit (P1811).

Details

Specifications

Features	Specifications
Main Functions	Isolation up to 20µg plasmid DNA from 1.5ml bacterial culture using 96 well bind plate and 96 filterplate
Applications	Enzyme digestion, sequencing, PCR, labeling, etc.
Purification method	96 well plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid, plasmid less than 30KB
Sample amount	1-1.5ml（x96）
Yield	1-15µg/1ml
Elution volume	≥70μl
Time per run	≤60 minutes
Liquid carrying volume per column	800µl
Binding yield of column	70µg

Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Kit Contents

Contents	P100601	P100602	P100603
Purification Times	1 x 96 Preps	4 x 96 Preps	20 x 96 Preps
RNase A	5 mg	20 mg	100 mg
Buffer P1	30 ml	120 ml	600 ml
Buffer P2	30 ml	120 ml	600 ml
Buffer P3	40 ml	180 ml	800 ml
Buffer PW1	100 ml	500 ml	2 x 1000 ml
Buffer PW2	50 ml	2 x 100 ml	4 x 200 ml
Elution Buffer	150 ml	60 ml	300 ml
Lysate Clear Plate	1	4	20
HiPure DNA Plate	1	4	20
1.6 ml Collection Plate	1	4	20
0.5ml Elute Plate	1	4	20

Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.

Purchase Guide

For guidance on selecting between standard and low-endotoxin plasmid workflows based on copy number, culture input and processing format, please refer to the Plasmid DNA Kits Purchase Guide.

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P1006 HiPure Plasmid Plus 96 Kit.pdf Preview Down

CAT NO	PRODUCT NAME	SIZE	PRICE
P100601	HiPure Plasmid Plus 96 Kit	1 x 96 preps	$164.00
P100602	HiPure Plasmid Plus 96 Kit	4 x 96 preps	$497.00
P100603	HiPure Plasmid Plus 96 Kit	20 x 96 preps	$2,238.00

HiPure Plasmid Plus 96 Kit

Introduction

Details

Principle

Purchase Guide

Articles

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