Details
Workflow
Workflow Overview
The MagPure Plant RNA Kit uses a magnetic-particle workflow for purification of total RNA from simple plant and fungal samples. After liquid-nitrogen grinding, the sample is lysed with Buffer RL or Buffer PRC1, with β-mercaptoethanol or DTT added when required for antioxidant support. The lysate is clarified by centrifugation, and the RNA-containing supernatant is combined with Buffer MCB and magnetic beads to establish RNA binding. Bound RNA is then processed through magnetic separation, washing, on-bead DNase digestion, re-binding, further washing, drying and elution in RNase-free water.
Sample Handling Logic
This workflow is designed for laboratories that prefer magnetic-particle RNA purification or need a workflow compatible with plate-based manual or automated processing. Buffer RL is used for routine plant samples, while Buffer PRC1 provides an alternative for samples affected by secondary metabolites or lysate solidification. The main sample-dependent variation occurs during liquid-nitrogen grinding, lysis, lysate clarification and bead resuspension. Integrated on-bead DNase digestion provides DNA reduction within the magnetic workflow without requiring a separate column membrane digestion step.
Time and Workflow Characteristics
Under typical manual operation, the workflow is usually completed within about 80–95 minutes, depending mainly on sample disruption, lysate clarity, magnetic bead resuspension, separation efficiency, on-bead DNase digestion and drying control. This route is suitable for plant RNA extraction workflows requiring magnetic handling, plate-format processing or automation compatibility. For detailed step-by-step conditions, workflow guidance and estimated processing times, please refer to the Workflow Note in the Download section.
Specifications
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Features
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Specifications
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Main Functions
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Isolation total RNA from 50mg plant using magnetic particles
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Applications
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RT-PCR, cDNA synthesis, second generation sequencing
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Purification method
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Polydisperse magnetic beads
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Purification technology
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Magnetic beads technology
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Process method
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Manual or automatic
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Sample type
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Plant and fungus samples
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Sample amount
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≤50mg
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Yield
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2-100μg
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Time per run
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~80-95 minutes
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Technical Validation
MagPure Plant RNA Kit was evaluated as a magnetic bead-based RNA extraction workflow for plant samples, with emphasis on DNA removal, lysis-buffer compatibility and downstream automation readiness. The validation used 50 mg Epipremnum aureum leaf input, magnetic bead purification, 70 µL elution volume and Nanodrop plus agarose gel electrophoresis analysis.
In the DNase I treatment workflow, 50 mg of Epipremnum aureum leaf tissue was processed using both RLC and PAL lysis routes. RLC-based extraction produced RNA yields of 3.22–3.53 µg, with A260/280 values of 2.09–2.13 and A260/230 values of 1.86–1.94. PAL-based extraction produced RNA yields of 3.47–3.55 µg, with A260/280 values of 2.04–2.10 and A260/230 values of 1.86–1.98, indicating high-quality RNA suitable for downstream applications.
The comparison between RLC and PAL lysis showed no obvious difference in RNA yield or purity in the tested plant sample, supporting the use of alternative lysis routes depending on plant matrix behavior. This is consistent with the kit design, where Buffer RL is used as the routine lysis buffer and Buffer PRC1 / PAL-type lysis may be selected when plant secondary metabolites affect sample processing.
Agarose gel electrophoresis showed effective removal of genomic DNA after DNase I treatment, with no obvious DNA residue observed in the tested samples. Together with the magnetic particle workflow and KingFisher-compatible plate setup, these results support R6641 for routine plant RNA extraction where automation, reproducible magnetic handling and integrated DNA removal are required.
Kit Contents
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Contents
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R664101
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R664102
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R664103
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Purification Times
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48 Preps
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96 Preps
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480 Preps
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MagPure RNA Particles
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1.7 ml
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3.5 ml
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18 ml
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DNase I
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600 μl
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2 x 600 μl
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10 x 600 μl
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DNase Buffer
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30 ml
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40 ml
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200 ml
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Buffer PRC1
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40 ml
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70 ml
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350 ml
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Buffer RL
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40 ml
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70 ml
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350 ml
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Buffer MCB*
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18 ml
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30 ml
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150 ml
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Buffer MW1*
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22 ml
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44 ml
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220 ml
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Buffer MW2*
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20 ml
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50 ml
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2 x 100 ml
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RNase Free Water
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10 ml
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15 ml
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120 ml
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Storage and Stability
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.
