| CAT NO | PRODUCT NAME | SIZE | PRICE |
| R415102 | HiPure Plant RNA Kit | 50 preps | $149.00 |
| R415103 | HiPure Plant RNA Kit | 250 preps | $622.00 |
The HiPure Plant RNA Mini Kit (R4151) is designed for routine RNA extraction from plant tissues with low levels of polysaccharides and polyphenols.
The workflow uses guanidine-based lysis combined with dual-column purification for RNA isolation and genomic DNA removal.
The HiPure Plant RNA Mini Kit uses a dual-column silica workflow for purification of high-quality RNA from simple plant samples. Plant tissue is first disrupted under liquid nitrogen and lysed with Buffer RLC or Buffer PRC1 in the presence of β-mercaptoethanol or DTT. The clarified lysate is passed through a gDNA removal column, where genomic DNA is reduced while the RNA-containing flow-through is collected. Ethanol is then added to the flow-through to establish RNA binding conditions, and the sample is loaded onto the RNA column for binding, washing, drying and elution.
This workflow is designed for routine plant RNA extraction where operational simplicity and upstream gDNA reduction are important. Buffer RLC is used for most simple plant samples, while Buffer PRC1 provides an alternative for samples that may be affected by secondary metabolites or lysate solidification. The main sample-dependent variation occurs during liquid-nitrogen grinding, lysate clarification and passage through the gDNA removal column. Once the RNA-containing flow-through has been adjusted with ethanol, the downstream column workflow remains consistent.
Under typical manual operation, the workflow is usually completed within about 35–45 minutes, depending mainly on sample disruption, lysate clarity and loading volume. Optional on-column DNase digestion can be added when downstream applications require more stringent DNA control. This route is suitable for laboratories that need a straightforward dual-column plant RNA workflow with gDNA reduction before RNA purification. For detailed step-by-step conditions, workflow guidance and estimated processing times, please refer to the Workflow Note in the Download section.
| Features | Specifications |
| Main Functions | Isolation total RNA from <150 mg simple plant sample without chloroform |
| Applications | RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation |
| Purification method | Mini spin column |
| Purification technology | Silica technology, DNA filtration technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Economic crops |
|
Sample amount |
≤150 mg |
| Elution volume | ≥30μl |
| Time per run | ~35-45 minutes |
| Liquid carrying volume per column |
800µl |
| Binding yield of column | 100µg |
| Contents | R415102 | D415103 |
| Purification Times | 50 Preps |
250 Preps |
|
HiPure DNA Mini Column II |
50 |
250 |
| HiPure RNA Mini Columns | 50 |
250 |
| 2ml Collection Tubes | 100 |
2 x 250 |
|
Buffer RLC |
50 ml |
200 ml |
|
Buffer PRC1 |
50 ml |
200 ml |
| Buffer RW1 | 50 ml |
200 ml |
|
Buffer RW2* |
20 ml |
2 x 50 ml |
|
RNase Free Water |
10 ml |
30 ml |
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
HiPure Plant RNA Mini Kit (R4151) is suitable for routine plant samples with low polysaccharide and polyphenol content.
For unknown or variable samples, HiPure Plant RNA Plus Kit (R4150) is recommended as a more broadly compatible system.
For difficult plant samples, CTAB-based workflows such as HiPure HP Plant RNA Mini Kit (R4165) may provide improved performance.
For a complete selection guide:
D3124
R4150
D6359D
R6672C
IVD3102
R4111
R6327
D3126
IVD5435
IVD3182
D3111
