HiPure Fruit RNA Kit

The HiPure Fruit RNA Kit (R4014) is designed for RNA extraction from fruit and starch-containing plant samples.

The workflow combines phenol–chloroform phase separation with column purification, enabling RNA recovery from samples where polysaccharides and storage compounds interfere with standard methods.

Specifications

Principle

The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 15 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 300µl water using the Kit.

Technical Validation

HiPure Fruit RNA Kit was evaluated using fruit and complex plant tissue samples to assess RNA extraction performance from matrices that may contain starch, polysaccharides, polyphenols, pigments and other plant-derived inhibitors. The validation compared the PlantZol-based R4014 workflow with a MagZol / Trizol-type reference workflow, followed by silica column purification, Nanodrop measurement and agarose gel electrophoresis analysis.

In fruit sample testing, R4014 was evaluated with orange, pear, guava, tomato, melon, dragon fruit and grape samples using 100 mg input. Under the tested conditions, From 100 mg fruit tissue input, R4014 produced measurable RNA from all tested fruit samples, including 3.62–4.78 µg from orange, 2.08–2.42 µg from pear, 3.10–3.44 µg from guava, 2.39–2.50 µg from tomato, 2.25–4.60 µg from melon, 8.48–10.40 µg from dragon fruit and 0.84–2.15 µg from grape. In several fruit matrices, especially guava and dragon fruit, the PlantZol-based workflow showed better recovery behavior than the MagZol / Trizol-type reference workflow.

The kit was further tested with plant leaf samples, including Pachira macrocarpa, Ficus, mulberry, Alocasia, bamboo, pepper and banana leaves. From 100 mg plant leaf input, R4014 produced RNA yields of 59.76–60.50 µg from Pachira macrocarpa leaves, 25.48–26.33 µg from Ficus leaves. In Ficus, mulberry and Alocasia leaf samples, R4014 showed higher or more consistent RNA recovery than the reference MagZol workflow under the tested conditions.

Electrophoresis analysis supported RNA recovery from both fruit and leaf samples, while the PlantZol lysis and column purification workflow provided a structured route for processing inhibitor-rich plant materials. The purified RNA is suitable for routine downstream applications such as RT-PCR, Northern blotting, poly(A)⁺ RNA purification, nuclease protection and in vitro translation. For highly variable plant tissues, sample input and homogenization should be optimized to maintain stable RNA yield and purity.

Kit Contents

Storage and Stability

The Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.

HiPure Fruit RNA Kit (R4014) is suitable for fruit samples and plant tissues containing starch or storage components.

For general plant samples, HiPure Plant RNA Mini Kit (R4151) may be used.

For difficult plant samples with high inhibitor content, HiPure HP Plant RNA Mini Kit (R4165) can be considered.

For a complete selection guide:

👉 Plant DNA and RNA Extraction Selection Guide