| CAT NO | PRODUCT NAME | SIZE | PRICE |
| R401402 | HiPure Fruit RNA Kit | 50 preps | $149.00 |
| R401403 | HiPure Fruit RNA Kit | 250 preps | $622.00 |
The HiPure Fruit RNA Kit (R4014) is designed for RNA extraction from fruit and starch-containing plant samples.
The workflow combines phenol–chloroform phase separation with column purification, enabling RNA recovery from samples where polysaccharides and storage compounds interfere with standard methods.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from <150 mg fruit or seed using chloroform and Plantzol reagent |
| Applications | RT-PCR, qPCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation translation |
| Purification method | Mini spin column |
| Purification technology | Silica technology, DNA filtration technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Simple plant, petal, peel, seed |
|
Sample amount |
50-150mg |
| Elution volume | ≥300μl |
| Time per run | ≤15 minutes（1-24 samples） |
| Liquid carrying volume per column |
800µl |
| Binding yield of column | 100µg |
The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 15 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 300µl water using the Kit.
Technical Validation
Comparative experiments showed:
· Stable RNA recovery from fruit samples using phenol-based extraction
· Improved yield compared to standard workflows in starch-containing matrices
Kit Contents
| Contents | R401402 | D401403 |
| Purification Times | 50 Preps |
250 Preps |
| HiPure RNA Mini Columns | 50 |
250 |
| 2ml Collection Tubes | 50 |
250 |
|
PlantZol Reagent |
60 ml |
200 ml |
|
Buffer GXP |
30 ml |
150 ml |
| Buffer RW1 | 50 ml |
200 ml |
|
Buffer RW2* |
20 ml |
2 x 50 ml |
|
RNase Free Water |
10 ml |
30 ml |
Storage and Stability
The Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
HiPure Fruit RNA Kit (R4014) is suitable for fruit samples and plant tissues containing starch or storage components.
For general plant samples, HiPure Plant RNA Mini Kit (R4151) may be used.
For difficult plant samples with high inhibitor content, HiPure HP Plant RNA Mini Kit (R4165) can be considered.
For a complete selection guide:
D3124
R4150
D6359D
R6672C
IVD3102
R4111
R6327
D3126
IVD5435
IVD3182
D3111
