Introduction

The HiPure Gel DNA Kit is designed for recovery of DNA fragments from agarose gels following electrophoresis. The workflow combines controlled gel dissolution conditions with silica membrane-based purification to ensure efficient DNA adsorption and reproducible recovery.

This system is typically used in cloning, sequencing and enzymatic workflows where removal of agarose and contaminants is required. The purification chemistry is optimized to maintain stable performance across different fragment sizes and routine laboratory conditions.

Details

Specifications

Features	Specifications
Main Functions	Recover DNA fragments >100bp from agarose gel(<0.5g), purification of DNA from PCR, enzymatic reaction solution or crude gDNA
Applications	PCR, NGS, labeling, ligation and enzyme digestion, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Agarose gel, PCR products, enzyme products
Sample amount	Agarose gel: ≤500mg
Recovery	≥80%
Elution volume	≥15μl
Time per run	≤20 minutes（1-24 samples）
Liquid carrying volume per column	800µl
Binding yield of column	35µg

Principle

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

High recovery efficiency - ≥80% DNA recovery
General - recover DNA from gel or enzyme-driven reaction solutions such as PCR
Fast – isolation can be completed in 10-15 minutes by column gel method
Great cost-effectiveness performance

Kit Contents

Contents	D211102	D211103
Purification Times	100 Preps	250 Preps
Buffer GDP	120 ml	250 ml
Buffer DW2	50 ml	2 x 50 ml
Elution Buffer	20 ml	30 ml
HiPure DNA Mini Columns II	100	250
2 ml Collection Tubes	100	250

Storage and Stability

The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.

Experiment Data

Purchase Guide

For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:

👉 Magen Kits Selection Guide

Articles

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D2111 HiPure Gel DNA Mini Kit.pdf Preview Down

CAT NO	PRODUCT NAME	SIZE	PRICE
D211102	HiPure Gel DNA Mini Kit	100 preps	$60.00
D211103	HiPure Gel DNA Mini Kit	250 preps	$150.00

HiPure Gel DNA Mini Kit

Introduction

Details

Principle

Purchase Guide

Articles

Download

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