| CAT NO | PRODUCT NAME | SIZE | PRICE |
| D212002 | HiPure PCR Pure Micro Kit | 100 preps | $60.00 |
| D212003 | HiPure PCR Pure Micro Kit | 250 preps | $150.00 |
The HiPure PCR Purification Kit is designed for cleanup of DNA from PCR and enzymatic reactions. The system removes primers, nucleotides, enzymes and salts, allowing purified DNA to be directly used in downstream applications.
The workflow is optimized for routine molecular biology applications where stable recovery and consistent purification performance are required without additional processing steps.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments between 60bp-20kbp from PCR products and enzymatic reaction solution |
| Applications | PCR, sequencing, labeling reactions, ligations and restriction digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | PCR products, enzymatic reaction solution |
|
Sample amount |
10-200μl |
| Recovery | ≥80% |
| Elution volume | ≥7μl |
| Time per run | ≤10 minutes |
| Liquid carrying volume per column |
800µl |
| Binding yield of column | 10µg |
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
| Contents | D212002 | D212003 |
| Purification Times | 100 Preps |
250 Preps |
| Buffer DP | 60 ml |
120 ml |
| Buffer DW2 | 20 ml |
50 ml |
|
Elution Buffer |
15 ml |
30 ml |
|
HiPure DNA Micro Columns |
100 |
250 |
| 2 ml Collection Tubes | 100 |
250 |
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:
D6381
D3141F
D3124
R4150
D6359D
R6672C
IVD4179
R4165
IVD3102
D3018
IVD5412
IVD4175
R6611
R4310
IVD3020
R4111
R6327
D6364
D3142
D3146
D6351
D3187
D3126
IVD5435
IVD3182
D6311
D3111
