| CAT NO | PRODUCT NAME | SIZE | PRICE |
| D214102 | HiPure DNA Clean Up Kit | 100 preps | $60.00 |
| D214103 | HiPure DNA Clean Up Kit | 250 preps | $150.00 |
The HiPure DNA Clean Up Kit is designed for purification and concentration of DNA from a wide range of molecular workflows. The system supports DNA fragment recovery across a broad size range and can be directly applied to PCR products, enzymatic reactions and other DNA samples.
This kit is typically used as a flexible solution for routine cleanup when sample types vary across workflows and consistent purification performance is required.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments between 20bp-20kbp from crude DNA and enzymatic reaction solution |
| Applications | PCR, sequencing, labeling reactions, ligations and restriction digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Crude DNA, enzymatic reaction solution |
|
Sample amount |
<150μl |
| Recovery | 80% |
| Elution volume | ≥15μl |
| Time per run | ≤10 minutes |
| Liquid carrying volume per column |
800µl |
| Binding yield of column | 20µg |
The HiPure system uses a simple bind-wash-elute procedure. Gelslices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
| Contents | D214102 | D214103 |
| Purification Times | 100 Preps |
250 Preps |
| Buffer GWP | 30 ml |
60 ml |
| Buffer DW2* | 20 ml |
50 ml |
|
Elution Buffer |
30 ml |
60 ml |
|
HiPure DNA Mini Columns I |
100 |
250 |
| 2 ml Collection Tubes | 100 |
2 x 125 |
Storage and Stability
The Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:
D6381
D3141F
D3124
R4150
D6359D
R6672C
IVD4179
R4165
IVD3102
D3018
IVD5412
IVD4175
R6611
R4310
IVD3020
R4111
R6327
D6364
D3142
D3146
D6351
D3187
D3126
IVD5435
IVD3182
D6311
D3111
