| CAT NO | PRODUCT NAME | SIZE | PRICE |
| C13110 | HiPure DNA Mini Column II | 1000/Bag | $360.00 |
Mini Column II extends the standard column format by increasing membrane layers to improve binding capacity. This makes it more suitable for plasmid preparation and workflows where DNA input or expected yield is higher than routine levels.
In comparison to the basic mini format, it provides better tolerance to sample loading variability while maintaining stable purification performance.
Specifications
| Features | Specifications |
| Recommended application | Plasmid Mini Preparation, gDNA/ RNA Extraction, DNA/RNA Clean Up |
| Preservation conditions | Room temperature |
| Stability | Up to 4 years |
| Filter membrane | High quality glass fiber filter GF/B, 4 layers |
| Membrane aperture | 1.0μm |
| Maximum binding yield of plasmid | 35 μg |
|
Maximum yield of alcohol mediated Binding |
200 μg |
|
Single liquid carrying capacity of column |
800 μl |
|
Minimum elution volume |
50 μl |
|
Withstand centrifugal force |
16,000 x g |
|
Centrifuge |
Small high speed centrifuge (2ml) |
Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silicagel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
Ordering information
| CAT.No. |
Product Name |
Package |
| C13110 |
HiPure DNA Mini Column II (4 x GF/B) with 2ml Collection Tubes |
1000/Bag |
HiPure DNA Mini Column II (C13110) is designed for routine DNA purification across a wide range of biological samples and laboratory workflows.
For low-input or highly diluted samples, HiPure DNA Nano Column (C13010) or HiPure DNA Micro Column (C13011) may improve recovery efficiency.
For larger sample volumes or higher DNA yield requirements, HiPure DNA Midi Column (C13120) or HiPure DNA Maxi Column (C13122) can be considered.
For full comparison across column formats:
D3111
R4111
R6327
D3126
IVD5435
IVD3182
IVD3102
D3124
R4150
D6359D
R6672C
