Serum miRNA Extraction Kit | Plasma & Body Fluids

Introducción

The HiPure Serum miRNA Kit provides a column-based workflow for purification of cell-free total RNA, primarily miRNA and other small RNA from serum, plasma, urine and other cell-free body fluid samples. The protocol uses chaotropic lysis followed by selective protein precipitation and silica membrane purification.

The workflow enables rapid microRNA extraction from cell-free body fluids without phenol-based reagents and supports routine RT-PCR analysis and microRNA detection workflows.

For automated serum microRNA purification workflows laboratories may refer to the MagPure Serum miRNA Kit (R6628), while enrichment-based miRNA purification workflows are supported by the HiPure Universal miRNA Kit (R4310).

Detalles

Specifications

Features	Specifications
Main Functions	Isolation cell-free total RNA, primarily miRNA and other small RNA from 0.3-0.9mL cell-free body fluids such as serum, plasma, urine and secretion samples
Applications	RT-PCR, Northern blotting, poly(A)+ RNA purification, nuclease protection assays, in vitro translation
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	cell-free body fluids such as serum, plasma, urine and secretion samples
Sample amount	0.3–0.9 mL
Minimum Elution Volume	20 µL
Time per run	Within 45 minutes
Maximum Column Loading Volume	800 µL
Maximum Nucleic Acid Binding Capacity	100 µg

Kit Contents

Contents	R431402	R431403
Purification Times	50 Preps	250 Preps
HiPure Viral Mini Columns	50	250
2ml Collection Tubes	100	4 x 125
Buffer CFL	20 ml	80 ml
Buffer CFP	6 ml	30 ml
Buffer MGW1*	30 ml	150 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	30 ml

Storage and Stability

The kit components can be stored at room temperature (15-25°C) and are stable for at least 24 months under these conditions.

Guía de Compra

Choosing the appropriate RNA extraction system depends on sample type, RNA species and workflow requirements.

For detailed selection guidance across blood and liquid RNA workflows, refer to the Blood RNA Kits Selection Guide.

Artículos

Descargar

R4314 HiPure Serum Plasma miRNA Kit 202608.pdf Vista previa Abajo

CAT NO	PRODUCT NAME	SIZE	PRICE
R431402	HiPure Serum/Plasma miRNA Kit	50 preps	$149.00
R431403	HiPure Serum/Plasma miRNA Kit	250 preps	$646.00

HiPure Serum/Plasma miRNA Kit

Introducción

Detalles

Guía de Compra

Artículos

Descargar

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