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MagPure Fast Blood DNA Kit D6310

CAT NO PRODUCT NAME SIZE PRICE
D631001 MagPure Fast Blood DNA Kit 48 preps $119.00
D631002 MagPure Fast Blood DNA Kit 96 preps $224.00
D631003 MagPure Fast Blood DNA Kit 480 preps $895.00

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting.Total DNA(e.g., genomic, viral, mitochondrial) can be purified from wholeblood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes,cultured cells.

Details


Specifications

Features Specifications
Main Functions Isolation total DNA from 200μl whole blood
Applications PCR, southern bolt and virus detection, etc
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Whole blood, concentrated blood, buffy coat, andother samples
Sample amount 200μl
Elution volume ≥70μl
Time per run ≤40 minutes


Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

  • High binding force - suitable for handling DNA rich samples, such as whole blood, buffy coat, concentrated blood, etc.
  • Fast - polydisperse magnetic beads, fast magnetic response and short extraction time
  • High purity - the obtained DNA can be directly used for second-generation sequencing, PCR based detection, gene bank, etc.

Kit Contents

Contents D631001 D631002 D631003
Purification Times 48 96 480
MagPure Particles 1.7 ml 3.5 ml 17 ml
Proteinase K 24 mg 50 mg 220 mg
Protease Dissolve Buffer
1.8 ml
3 ml
15 ml
Buffer MLA 30 ml 60 ml 270 ml
Buffer DW1 60 ml 120 ml 550 ml
Buffer CW 30 ml 60 ml 270 ml
Buffer BW3 30 ml 60 ml 270 ml
Elution Buffer 15 ml 30 ml 100 ml

Storage and Stability

Proteinase K, MagPureParticles should be stored at 2-8°C upon arrival. However, short-term storage(up to 24 weeks) at room temperature (15-25°C) does not affect theirperformance. The remaining kit components can be stored at room temperature (15-25°C)and are stable for at least 18 months under these conditions.

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