Low-Endotoxin Plasmid DNA Maxi Prep Kit for Transfection

Introduction

HiPure Plasmid EF Maxi Kit is the earliersupport configuration within the Magen low-endotoxin plasmid maxi branch. Itremains suitable for routine low-endotoxin plasmid preparation in aconventional column format, while HiPure Plasmid EF Maxi Kit B (P1156B) servesas the upgraded core product for broader workflow compatibility and improvedendotoxin and RNA control.

Details

Specifications

Features	Specifications
Main Functions	Isolation up to 1.5mg endotoxin-free plasmid DNA from 200ml bacterial culture
Applications	Cell transfection, animal injection, etc.
Purification method	Maxi spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid vector
Sample amount	High copy plasmid vector: 100-150ml culture medium Low copy plasmid vector: 150-200ml culture medium
Yield	200-1500μg
Elution volume	≥0.7ml
Time per run	≤60 minutes
Liquid carrying volume per column	20ml
Binding yield of column	1mg

Kit Contents

Contents	P115602	P115603
Purification Times	10 Preps	50 Preps
RNase A	30 mg	150 mg
Buffer P1	100 ml	500 ml
Buffer P2	100 ml	500 ml
Buffer LN3	50 ml	250 ml
Buffer PW1	33 ml	180 ml
Buffer PW2	20 ml	100 ml
Elution Buffer	15 ml	120 ml
Buffer CL	33 ml	180 ml
HiPure DNA Maxi Columns C	10	50
Lysate Clear Midi Syringe	10	50
50 ml Collection Tubes C	20	100

Storage and Stability

The kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2–8°C.

Purchase Guide

For guidance on selecting between standard and low-endotoxin plasmid workflows based on copy number, culture input and processing format, please refer to the Plasmid DNA Kits Purchase Guide.

CAT NO	PRODUCT NAME	SIZE	PRICE
P115602	HiPure Plasmid EF Maxi Kit	10 preps	$164.00
P115603	HiPure Plasmid EF Maxi Kit	50 preps	$746.00

HiPure Plasmid EF Maxi Kit

Introduction

Details

Purchase Guide

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