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MagPure Universal DNA Kit IVD3102

CAT NO PRODUCT NAME SIZE PRICE
IVD3102 MagPure Universal DNA Kit 200 preps $448.00

Introduction

This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Details


Specifications

Features Specifications
Main Functions Isolation total DNA from blood, buffy coat, tissue and other samples
Applications Second generation sequencing, PCR, real time PCR, etc.
Purification method
Polydisperse magnetic beads
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
Sample amount Whole blood :< 200μl; Saliva / swab:< 400μl; Tissue :< 20mg
Yield 0.1 - 50μg
Elution volume
Time per run


Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

  • High purity - OD 260 / 280 : 1.7- 1.9, OD 260 / 230 : 1.5 - 2.0
  • Economy - less than 50% of the price of Qiagen and other imported products
  • High yield - most optimal process, ensuring the recovery up to 90%
  • Strong processing ability - samples including animal blood, cultured cells, animal tissues, etc.


Kit Contents

Contents IVD3102
Purification Times 200
MagPure Particles 6.6 ml
Proteinase K 90 mg
Protease Dissolve Buffer 10 ml
Rnase A 40 mg
Buffer ATL 60 ml
Buffer AL 60 ml
Buffer BD*
20 ml
Buffer BW1*
110 ml
Elution Buffer 30 ml

Storage and Stability

Proteinase K, RNase A, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.


Experiment Data


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