| CAT NO | PRODUCT NAME | SIZE | PRICE |
| P115602 | HiPure Plasmid EF Maxi Kit | 10 preps | $164.00 |
| P115603 | HiPure Plasmid EF Maxi Kit | 50 preps | $746.00 |
HiPure Plasmid EF Maxi Kit is the earliersupport configuration within the Magen low-endotoxin plasmid maxi branch. Itremains suitable for routine low-endotoxin plasmid preparation in aconventional column format, while HiPure Plasmid EF Maxi Kit B (P1156B) servesas the upgraded core product for broader workflow compatibility and improvedendotoxin and RNA control.
| Features | Specifications |
| Main Functions | Isolation up to 1.5mg endotoxin-free plasmid DNA from 200ml bacterial culture |
| Applications | Cell transfection, animal injection, etc. |
| Purification method | Maxi spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
|
Sample type |
Conventional plasmid vector |
| Sample amount |
High copy plasmid vector: 100-150ml culture medium
Low copy plasmid vector: 150-200ml culture medium |
| Yield | 200-1500μg |
| Elution volume | ≥0.7ml |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 20ml |
| Binding yield of column | 1mg |
The HiPure Plasmid EF Maxi Kit was validated using high-copy and low-copy plasmid cultures to evaluate plasmid yield, purity and extraction scalability. In the test, high-copy vector cultures were processed from 50–100 mL input volumes, while low-copy vector cultures were processed from 100–200 mL input volumes. Purified plasmid DNA was analyzed by NanoDrop measurement and agarose gel electrophoresis.
Across the tested samples, plasmid DNA purified using P1156 showed A260/280 values of approximately 1.79–1.90 and A260/230 values of approximately 1.8–2.5, indicating suitable purity for routine molecular biology applications. For high-copy plasmid cultures, P1156 produced approximately 0.75–0.89 mg plasmid DNA from 50 mL culture and up to approximately 1.1–1.4 mg from 100 mL culture under the tested conditions.
For low-copy plasmid cultures, P1156 produced approximately 250–550 µg plasmid DNA from 100–200 mL bacterial culture. The extraction efficiency was comparable to conventional small-scale plasmid extraction when normalized by culture volume, supporting the use of P1156 for larger-volume plasmid preparation where increased total DNA output is required.
Additional testing showed that the PW1 wash step can reduce RNA-related overestimation and produce more realistic plasmid yield readings, especially when processing low-copy plasmid cultures. The results also indicate that culture volume should be selected according to plasmid copy number and column binding capacity, as excessive high-copy culture input may exceed the binding capacity and reduce extraction efficiency.
| Contents | P115602 | P115603 |
| Purification Times | 10 Preps |
50 Preps |
| RNase A | 30 mg |
150 mg |
| Buffer P1 | 100 ml |
500 ml |
|
Buffer P2 |
100 ml |
500 ml |
|
Buffer LN3 |
50 ml |
250 ml |
|
Buffer PW1 |
33 ml |
180 ml |
| Buffer PW2 | 20 ml |
100 ml |
| Elution Buffer | 15 ml |
120 ml |
| Buffer CL | 33 ml | 180 ml |
|
HiPure DNA Maxi Columns C |
10 |
50 |
|
Lysate Clear Midi Syringe |
10 |
50 |
|
50 ml Collection Tubes C |
20 |
100 |
The kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2–8°C.
For guidance on selecting between standard and low-endotoxin plasmid workflows based on copy number, culture input and processing format, please refer to the Plasmid DNA Kits Purchase Guide.
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