Introduction

This Kit provides a vacuum-compatible configuration within the Magen membrane-based circulating DNA platform. It supports 1–5mL plasma purification using vacuum-driven filtration.

This format is suitable for laboratories operating vacuum manifolds while maintaining compatibility with the core membrane chemistry.

For the primary column reference model, see HiPure Circulating DNA Kit – IVD3182.

For centrifuge-based processing, refer to HiPure Circulating DNA Midi Spin Kit – D3182D.

This product is for research use only.

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Details

Specifications

Features	Specifications
Main Functions	Isolation circulating DNA from 1-5ml plasma, serum, body fluids using vacuum protocol
Applications	qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (vacuum)
Sample type	Serum, plasma and other cell-free fluid samples
Sample amount	1-5ml
Elution volume	≥50μl
Time per run	≤60 minutes
Liquid carrying volume per column	4ml
Binding yield of column	1mg

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer.

Advantages

High yield - most optimal process, free DNA (>50bp) can be obtained to the maximum extent
High concentration - low elution volume, ensuring high nucleic acid concentration
High purity - low alcohol binding method, completely removing inhibitor and protein pollution
High recovery - DNA can be recovered at thelevel of PG by silica gel column purification

For detailed engineering characteristics and validation data, refer to the core circulating DNA extraction system.

Kit Contents

Contents	D318201C	D318202C
Purification Times	10	50
Buffer ACL	50 ml	250 ml
Buffer ACB*	60 ml	300 ml
Buffer DCW1*	4.4 ml	22 ml
Buffer DCW2*	5 ml	10 ml
Proteinase K	110 ml	540 mg
Protease Dissolve Buffer	10 ml	30 ml
Carrier RNA	110 μg	110 μg
Nuclease Free Water	10 ml	20 ml
HiPure CFDNA Mini Columns	10	50
2 ml Collection Tubes	20	100
Extender Tube	10	50
Vac-Connector	10	50

Storage and Stability

Proteinase K, Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

Purchase Guide

Select the right purification kit to get impactful results：

Ⅰ. Purchase guide of Magen kits based on sample

Ⅱ. Cross purchase guide for Magen kits vs Other brands

For any customized product or OEM service, please contact us.

CAT NO	PRODUCT NAME	SIZE	PRICE
D318201C	HiPure Circulating DNA Midi Kit C	10 preps	$136.00
D318202C	HiPure Circulating DNA Midi Kit C	50 preps	$547.00

HiPure Circulating DNA Midi Kit C (Vaccum Protocol)

Introduction

Video

Details

Principle

Advantages

Kit Contents

Purchase Guide

Articles

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