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HiPure Plant DNA Mini Kit D3187

CAT NO PRODUCT NAME SIZE PRICE
D318702 HiPure Plant DNA Mini Kit 50 preps $99.00
D318703 HiPure Plant DNA Mini Kit 250 preps $448.00

Introduction

HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.

Details


Specifications

Features Specifications
Main Functions Isolation total DNA from 150 mg plant and fungal tissue
Applications PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Fresh / frozen plant samples, dried plant / seed samples
Sample amount

Fresh / frozen plant samples: 50-150 mg

Dried plant / seed samples: 15-40 mg

Elution volume ≥40μl
Time per run 30-50 minutes
Liquid carrying volume per column 800μl
Binding yield of column 100μg


Principle

This product is based on silica column purification. The sample is lysed with CATB Buffer. DNA is released into the lysate. Cell debris, precipitated proteins and polysaccharides are removed by chloroform extraction. After adjust the binding condition, transfer to an adsorption column. DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

  • Broad spectrum - suitable for extracting DNA from various plant samples
  • Fast - several samples can be extracted in 30 minutes by silica technology
  • Good repeatability - silica gel column purification technology can obtain ideal results every time


Kit Contents

Contents D318702 D318703
Purification Times 50 Preps 250 Preps
Buffer PAL 60 ml 200 ml
Buffer GWP 60 ml 200 ml
Buffer DW1
30 ml
150 ml
Buffer GW2* 20 ml 50 ml
Buffer AE
20 ml
60 ml
HiPure DNA Mini Columns II
50 250
2 ml Collection Tubes
50 250


Storage and Stability

This product can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

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