Introduction
The HiPure HP Plant DNA Kit (D3187) is designed for genomic DNA extraction from plant tissues with high levels of polysaccharides, polyphenols and secondary metabolites, where standard SDS-based workflows often result in low yield or co-purification of inhibitors.
This kit combines CTAB-based lysis with organic extraction and silica column purification to enable stable DNA recovery from complex plant matrices. The workflow is particularly suited for woody plants, mature tissues and starch-containing samples that require additional impurity removal capacity.
Within the Magen plant DNA system, D3187 serves as the primary solution for complex plant samples, while routine samples can be processed using D3164 and automated workflows are supported by D6351.
Details
Specifications
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Features
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Specifications
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Main Functions
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Isolation total DNA from 150 mg plant and fungal tissue
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Applications
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PCR, SSR, AFLP, RAPD and southern blot, etc.
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Purification method
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Mini spin column
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Purification technology
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Silica technology
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Process method
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Manual (centrifugation or vacuum)
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Sample type
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Fresh / frozen plant samples, dried plant / seed samples
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Sample amount
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Fresh / frozen plant samples: 50-150 mg
Dried plant / seed samples: 15-40 mg
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Elution volume
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≥40μl
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Time per run
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30-50 minutes
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Liquid carrying volume per column
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800μl
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Binding yield of column
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100μg
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Extraction Principle
Plant tissues are first lysed using a CTAB-based buffer system designed to disrupt cell walls and solubilize nucleic acids while preventing co-precipitation of polysaccharides.
Polyphenols and secondary metabolites are removed through organic extraction and selective phase separation, allowing DNA to remain in the aqueous phase.
Under high-salt conditions, genomic DNA binds to the silica membrane, while residual proteins, pigments and inhibitors are removed through sequential washing steps. Purified DNA is then eluted for downstream applications.
Engineering Features
CTAB-based lysis for inhibitor-rich samples
Enables disruption of plant cell walls while reducing co-extraction of polysaccharides and polyphenols that interfere with downstream enzymatic reactions.
Organic extraction for impurity partitioning
Phase separation improves removal of pigments and secondary metabolites, which are not efficiently cleared in detergent-only systems.
Silica membrane DNA purification
Provides consistent DNA recovery across different plant species and sample types.
Adapted for high-complexity plant matrices
Designed for samples where conventional rapid extraction methods are insufficient.
Technical Validation
Validation across multiple plant species and sample types showed:
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Intact genomic DNA with clear electrophoretic band patterns
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Consistent DNA yield across replicate extractions
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Successful PCR amplification from both fresh plant tissues and processed seed samples without detectable inhibition
These results indicate effective removal of plant-derived inhibitors that commonly affect downstream applications.
Kit Contents
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Contents
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D318702
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D318703
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Purification Times
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50 Preps
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250 Preps
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Buffer PAL
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60 ml
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200 ml
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Buffer GWP
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60 ml
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200 ml
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Buffer DW1
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30 ml
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150 ml
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Buffer GW2*
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20 ml
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50 ml
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Buffer AE
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20 ml
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60 ml
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HiPure DNA Mini Columns II
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50
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250
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2 ml Collection Tubes
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50
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250
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Storage and Stability
This product can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Purchase Guide
HiPure HP Plant DNA Kit (D3187) is designed for plant samples with high levels of polysaccharides and polyphenols, where standard extraction methods may result in low DNA yield or purity.
For routine plant tissues with low inhibitor content, SDS-based workflows such as HiPure SF Plant DNA Kit (D3164) may be sufficient.
For automated or high-throughput workflows, magnetic bead–based systems such as MagPure Plant DNA Kit (D6351) can be considered.
For a complete selection guide:
