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MagPure Universal RNA Kit IVD3020

CAT NO PRODUCT NAME SIZE PRICE
IVD3020 MagPure Universal RNA Kit 200 preps $895.00

Introduction

This kit is suitable for high-throughput extraction of total RNA from cultured cells, cell suspensions, animal tissues, conventional fungi and plant tissues. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 60 minutes. The obtained RNA can be directly used in RT-PCR, fluorescence quantification, second-generation sequencing and viral RNA detection and so on. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 hole oscillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.

Details


Specifications

Features Specifications
Main Functions Isolation total RNA from tissue, cell
Applications RT-PCR, cDNA synthesis, second generation sequencing
Purification method Polydisperse magnetic beads
Purification technology Magnetic beads technology
Process method Manual or automatic
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Sample type Tissues, cells, lymphocytes and other clinical sample
Sample amount

Cells grown insuspension:1 x 107

Animal tissue: 20mg

Plant tissue: 100mg

Whole blood: 1.5ml

Yield 2-50μg


Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.


Advantages

  • High purity - OD260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
  • Economy - less than 50% of the price of Qiagen and other imported products
  • Automatic - extraction without manual participation, saving time and effort
  • Universal - suitable for various clinical samples


Kit Contents

Contents IVD3020
Purification Times 200 Preps
MagPure RNA Particles
7 ml
DNase I
4 x 600 µl
DNase Buffer
80 ml
RTL Lysis Buffer
150 ml
Buffer MCB
75 ml
Buffer MW1 88 ml
Buffer MW2
50 ml
RNase Free Water
60 ml


Storage and Stability

This product can be stored at room temperature (15-25ºC) for 12 months. MagPure RNA Particles, DNase I are shipped at room temperature. After received, store DNase I at -20ºC and store MagPure RNA Particles and proteinase K at 2-8ºC.

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