This kit is suitable for high-throughput extraction of total RNA from cultured cells, cell suspensions, animal tissues, conventional fungi and plant tissues. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation. The whole extraction process only takes 60 minutes. The obtained RNA can be directly used in RT-PCR, fluorescence quantification, second-generation sequencing and viral RNA detection and so on. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 hole oscillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.