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HiPure Viral DNA/RNA Kit IVD4173

CAT NO PRODUCT NAME SIZE PRICE
IVD4173 HiPure Viral RNA/DNA Kit 100 preps $249.00

Introduction

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

Details


Specifications

Features Specifications
Main Functions Extract viral RNA/DNA from 200μl plasma/serum samples
Applications RT-PCR,PCRNGS
Products Viral total nucleic acid, body cell total nucleic acid, negative bacterial DNA
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Whole blood, plasma, serum, soaking solution and tissue homogenate supernatant
Sample amount 200μl
Yield 2-10μg
Elution volume 30μl
Time per run 30 minutes
Liquid carrying volume per column 800μl
Binding yield of column 100μg


Principle

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.


Advantages

  • Fast - several samples can be extracted in 20 minutes by column method
  • High quality - high purity total RNA can be directly used in various sensitive downstream applications
  • Safe - no phenol chloroform extraction required
  • Sensitive - DNA/RNA can be recovered at the level of PG
  • High yield - carrier RNA contained in the product maximize the recovery of trace nucleic acid


Kit Contents

Contents IVD4173
Purification Times 100 Preps
HiPure Viral Mini Column 100
2ml Collection Tubes
200
PK/Carrier RNA 50 mg
Protease Dissolve Buffer 5 ml
Buffer AL
30 ml
Buffer MW1
44 ml
Buffer MW2
50 ml
RNase Free Water
15 ml


Storage and Stability

This kit is shipped and stored at room temperature and is valid for 12 months.


Experiment Data


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