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HiPure Blood DNA Mini Kit D3111

CAT NO PRODUCT NAME SIZE PRICE
D311102 HiPure Blood DNA Mini Kit 50 preps $99.00
D311103 HiPure Blood DNA Mini Kit 250 preps $472.00

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.

Details


Specifications

Features Specifications
Main Functions Isolation total DNA from 200ul Whole Blood
Applications PCR, southern bolt and virus detection, etc
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Blood, serum, plasma, milk, saliva, and other liquid samples and cultured cells
Sample amount 10-250ul
Elution volume ≥20μl
Time per run ≤30 minutes
Liquid carrying volume per column 800µl
Binding yield of column 100µg


Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

  • High quality DNA - meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
  • Fast - without separation of leukocytes, organic extraction or ethanol precipitation
  • Simple - all nucleic acids can be obtained by direct digestion
  • Wide applicability- handle a variety of liquid samples

Kit Contents

Contents D311102 D311103
Purification Times 50 250
HiPure DNA Mini Columns I 50 2 x 125
2ml Collection Tubes 100 5 x 100
Buffer AL 15 ml 60 ml
Buffer DW1 30 ml 150 ml
Buffer GW2 12 ml 50 ml
Proteinase K 24 mg 120 mg
Protease Dissolve Buffer 1.8 ml 10 ml
Buffer AE 15 ml 60 ml

Storage and Stability

Proteinase K should be stored at 2–8°Cupon arrival. However, short-term storage (up to 12 weeks) at room temperature(15–25°C) does not affect their performance. The remaining kit components canbe stored at room temperature (15–25°C) and are stable for at least 18 monthsunder these conditions.


Purchase Guide

Name CAT NO

Sample amount

Leukocyte protocol*

Colum type

Elutio volume

Average yield

Time per run

HiPure Blood DNA Mini Kit D3111 10-250μl 1ml 2ml column ≥20μl 5-9μg/200μl ≤30 minutes
HiPure Tissue&Blood DNA Midi Kit D3113 0.2-2ml 10ml 1.5ml column ≥300μl 20-40μg/1m ≤80 minutes
HiPure Tissue&Blood DNA Maxi Kit D3115 3 -10ml 10ml 15ml column ≥700μl 20-40μg/1m ≤90 minutes
HiPure Tissue&Blood DNA 96 Kit D3117 1-200μl 1ml 96 well plate
3-8μg/200μl

*Note:Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate, and white blood cells were obtained by centrifugation before extraction

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