Introduction

MagPure Circulating DNA Mini Kit is optimized for purification of circulating DNA from 0.2–0.6mL plasma using magnetic bead technology. It applies the same particle chemistry used in the Magen magnetic platform, scaled for lower input volumes.

This configuration is suitable for limited sample workflows or pilot studies requiring magnetic bead extraction at reduced plasma volumes.

For larger input volumes (1–8mL), refer to the magnetic core system MagPure Circulating DNA Maxi Kit – IVD5435

For membrane-based workflows, see HiPure Circulating DNA Kit – IVD3182

Video

Details

Specifications

Features	Specifications
Main Functions	Isolation circulating DNA from 0.2-0.6ml plasma, serum, body fluids
Applications	qPCR, NGS, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Serum, plasma
Sample amount	0.2-0.6ml
Elution volume	≥30μl
Time per run	≤50 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.

For detailed engineering characteristics and validation data, refer to the core circulating DNA extraction system.

To better understand how this product route has been used in published cfDNA / ctDNA research, please review the IVD5435 Application Scenario Summary .

Kit Contents

Contents	IVD5432
Purification Times	200
MagPure Particles G	14 ml
Carrier RNA	310 μg
Proteinase K	180 mg
Protease Dissolve Buffer	10 ml
Buffer MLK	250 ml
Buffer MAW1	250 ml
Buffer MW2*	2 x 50 ml
Elution Buffer	60 ml

Contents	IVD5432-F-96A	IVD5432-F-96B
Sample amount	200～350μl	400~700μl
Carrier RNA	110 μg	110 μg
Proteinase K	50 mg	100 mg
Protease Dissolve Buffer	5 ml	6 ml
Elution Buffer	15 ml	15 ml
Tip	1	1
Sample Plate A	500μl Buffer MLK	500μl Buffer MLK
Sample Plate B	/	500μl Buffer MLK
Wash Plate 1	700μl Buffer MAW1	700μl Buffer MAW1
Wash Plate 2	25μl Buffer MPG2 700μl Buffer MW2	25μl Buffer MPG2 700μl Buffer MW2
Wash Plate 3	700μl Buffer MW2	700μl Buffer MW2
Elution Plate	/	/

Contents	IVD5432-TL-06A	IVD5432-TL-06B	IVD5432-TL-06C
Sample amount	300~350μl	600~700μl	900~1050μL
Carrier RNA	310 μg	310 μg	310 μg
Proteinase K	50 mg	100 mg	150 mg
Protease Dissolve Buffer	6 ml	6 ml	10 ml
Elution Buffer	15 ml	15 ml	15 ml
DA-Tip	12	12	12
Row 1/7	600μl Buffer MLK	600μl Buffer MLK	600μl Buffer MLK
Row 2/8	/	600μl Buffer MLK	600μl Buffer MLK
Row 3/9	600μl Buffer MAW1	600μl Buffer MAW1	600μl Buffer MLK
Row 4/10	20μl Buffer MPG2 600μl Buffer MW2	20μl Buffer MPG2 600μl Buffer MW2	30μl Buffer MPG2 900μl Buffer MAW1
Row 5/11	600μl Buffer MW2	600μl Buffer MW2	900μl Buffer MW2
Row 6/12	/	/	/

Storage and Stability

Proteinase K, Carrier RNA and MagPure Particles G should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

Purchase Guide

For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:

👉 Magen Kits Selection Guide

For a broader technical overview of cfDNA workflow routes, processing logic and downstream application orientation:

👉 cfDNA Extraction & Enrichment Workflows

For reference workflow structure and comparative processing logic across representative cfDNA workflows:

👉 cfDNA Workflow Notes (IVD3182 / IVD5435 / 12927)

CAT NO	PRODUCT NAME	SIZE	PRICE
IVD5432	MagPure Circulating DNA Mini Kit	200preps	$398.00
IVD5432-TL-06A	MagPure Circulating DNA Mini Precast Kit (300μl)	96 preps	$265.00
IVD5432-TL-06B	MagPure Circulating DNA Mini Precast Kit (600μl)	96 preps	$332.00
IVD5432-TL-06C	MagPure Circulating DNA Mini Precast Kit (900μl)	96 preps	$398.00
IVD5432-F-96A	MagPure Circulating DNA Mini Precast Kit (300μl)	96 preps	$239.00
IVD5432-F-96B	MagPure Circulating DNA Mini Precast Kit (600μl)	96 preps	$299.00
IVD5432-F-96C	MagPure Circulating DNA Mini Precast Kit (900μl)	96 preps	$359.00

MagPure Circulating DNA Mini Kit

Introduction

Video

Details

Principle

Kit Contents

Purchase Guide

Articles

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