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Guanidine hydrochloride C11101

CAT NO PRODUCT NAME SIZE PRICE
C11101 Guanidine hydrochloride 5 KG $260.00
C11102 Guanidine hydrochloride 25 KG $999.00

Introduction

The product is the core raw material for nucleic acid extraction after strict screening and quality control. Guanidine hydrochloride is a powerful denaturantor ionizing agent, which can promote the solubility of hydrophobic molecules and denature proteins. It is also an effective denaturant and inactivator of avariety of enzymes and proteins. In 6 M guanidine hydrochloride solution, the ordered "secondary structure" (maintained by intermolecular non covalent interactions, such as hydrogen bonds and hydrophobic bonds) of almost all proteins is destroyed and the peptide chain is stretched. The tertiary structure has changed. In the process of nucleic acid extraction, guanidine hydrochloride is often used as cleaning solution (> 3M), binding solution (4-6M) for protein removal, and lysate for genomic DNA and total RNA extraction. Different from guanidine isothiocyanate, guanidine hydrochloride has low absorbance in A260, A280 and A230, and the ratio of nucleic acid product A260 /230 is more ideal.

Details


Specifications

Features
Specification
Basic content Recommended application
Plasmid extraction, lysis Solution or cleaning solution
CAS Numbers
50-01-1
Formula
CH5N3.HCL
Molecular Weight
95.53
Content
99.5%
Level
Molecular Biology
Appearance
White crystal, will harden for a long time
Transportation conditions
Room temperature
Preservation conditions
Room temperature, dry, dark
Impurity parameters
Moisture
≤0.3%
Ash
≤0.02%
Water insoluble matter
≤0.02%
Ammonium
≤0.05%
Ammonium dicyanide
≤0.1%
Ammonium cyanuric chloride
≤0.01%
Iron ≤0.1PPM
UV absorption value
Absorbance value @ 230 (6M)
≤0.4
Absorbance value @ 260 (6M)
≤0.04
Absorbance value @ 280 (6M)
≤0.01
Absorbance value @ 320 (6M)
≤0.01
Nucleic acid extraction related
Plasmid extraction test
Adopt
Blood DNA extraction test
Adopt
DNase test (2M)
Not detected
RNase test (2M)
Not detected
Purity of saturated solution (8M)
The 8m mother liquor is heated and dissolved. After dissolution, the insoluble matter is filtered with 3 layers of rapid filter paper.
PH value (6M)
5.0-5.5


Ordering information

CAT.No. Product Name
Package
C11101

Guanidine Hydrochloride

(Molecular Biology)

5KG
C11102
25KG (5KG/Bag, 5Bags/Bucket)

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