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Your present location:Home/Products/DNA&RNA Purification/RNA/Viral/Pathogen DNA/RNA/MagPure Pathogen DNA/RNA Kit B (mNGS)
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MagPure Pathogen DNA/RNA Kit B (mNGS)

R6672B
CAT NO PRODUCT NAME SIZE PRICE
R667202B MagPure Pathogen DNA/RNA Kit (mNGS) 96 preps $439.00

Introduction

This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples (including serum and plasma). The kit is based on super paramagnetic particles purification technology. Purified DNA/RNA is ready for downstream applications such as Real Time PCR, biochip analysis, NGS and other related experiments.

Details


Specifications

Features Specifications
Main Functions Extract Pathogen RNA/DNA from 0.5ml plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution for mNGS application, remove host background nucleic acid.
Applications RT-PCRReal Time PCR, biochip analysis, NGS
Products Pathogen DNA / RNA
Purification method Polydisperse magnetic beads
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type low/cell-free samples such as plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution
Sample amount 0.5 ml
Adaptive instrument
Nucleic acid extractor, pipetting workstation


Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.


Kit Contents

Contents R667200B R6672-02B
Purification Times 24 Preps 96 Preps
2ml Bead Tubes 24 96
Proteinase K 12 mg
50 mg
Protease Dissolve Buffer 1.8 ml 3 ml
Buffer SDS (20%)
1.8 ml
8 ml
MagBind Particles
0.6 ml
2.5 ml
Buffer MLB
15 ml
60 ml
Buffer MW1*
13 ml
44 ml
Buffer MW2*
6 ml
50 ml
Buffer AVE
5 ml
30 ml


Storage and Stability

MagBind Particles and Proteinase K Solution should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.

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