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HiPure Serum miRNA Kit

R4314
CAT NO PRODUCT NAME SIZE PRICE
R431402 HiPure Serum miRNA Kit 50 preps $149.00
R431403 HiPure Serum miRNA Kit
250 preps $646.00

Introduction

The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.


Details


Specifications

Features Specifications
Main Functions Isolation total RNA and miRNA from 0.2-0.6ml serum and plasma
Applications
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type
Sample amount
Yield
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column


Principle

HiPure RNA technology simplifies total RNA isolation. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After the removal of protein the binding conditions for nucleic acids are adjusted by adding isopropanol. Total nucleic acids are bound to the column. Optionally, DNA can be removed by an on-column DNase digest. The remaining nucleic acids are washed and eluted with minimal amounts of RNase-Free water.


Kit Contents

Contents R431402 R431403
Purification Times 50 Preps 250 Preps
HiPure RNA Mini Columns
100
2 x 250
2ml Collection Tubes 100 2 x 250
Buffer CFL
6 ml
30 ml
Buffer CFP 1.8 ml 10 ml
Buffer RWC
20 ml
80 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
60 ml


Storage and Stability

The kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

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