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MagPure Serum miRNA Kit

R6628
CAT NO PRODUCT NAME SIZE PRICE
R662801
MagPure Serum miRNA Kit
48 preps
$224.00
R662802 MagPure Serum miRNA Kit 96 preps $323.00
R662803 MagPure Serum miRNA Kit 5 x 96 preps $1,193.00

Introduction

The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Details


Specifications

Features Specifications
Main Functions Isolation miRNA from  0.3-0.5ml serum or plasma using magnetic particles
Applications RT-PCR, cDNA synthesis, second generation sequencing
Purification method Polydisperse magnetic beads
Purification technology Magnetic beads technology
Process method Manual or automatic
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Sample type Serum, plasma, acellular samples
Sample amount 300μl


Principle

This product is based on the purification method of high binding magnetic particles. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.


Advantages

  • Safe - no phenol chloroform extraction and no use of Trizol reagent
  • Fast - several samples can be extracted in 60 minutes by column method


Kit Contents

Contents R662801
R662802 R662803
Purification Times 48 Preps
96 Preps 5 x 96 Preps
MagPure Particles N 1.7 ml
3.5 ml 17 ml
Buffer CFL
6 ml
12 ml 60 ml
Buffer CPL
1.8 ml
3.5 ml
20 ml
Buffer MGW1* 30 ml
60 ml 250 ml
Buffer MW2*
12 ml
25 ml
100 ml
RNase Free Water
10 ml
20 ml
60 ml


Storage and Stability

MagPure Particle N should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

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