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HiPure Stool RNA Kit

R4185
CAT NO PRODUCT NAME SIZE PRICE
R418502 HiPure Stool RNA Kit 50 preps $298.00
R418503 HiPure Stool RNA Kit 250 preps $1,343.00

Introduction

Hipure Stool RNA Kit is specially designed for stool RNA extraction. This kit is suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples. The kit adopts silica gel column purification technology and original solution system, which can effectively remove humic acid and other inhibitory factors in stool samples. The purified RNA can be directly used in RT-PCR, Northern hybridization and other experiments.

Details


Specifications

Features Specifications
Main Functions Isolation total RNA from 100-150mg stool sample
Applications RT-PCR, Northern hybridization and other experiments
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Stool
Sample amount 100-150 mg
Elution volume ≥30μl
Time per run ≤50 minutes
Liquid carrying volume per column 100µg
Binding yield of column 800µl

Principle

The HiPure silica gel column uses a high binding ability glass fiber filter membrane as the substrate. Under the condition of high concentration of ionizing agent (such as Guanidinium chloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bonding and electrostatic and other physical factors, while protein or other impurities are not adsorbed and removed. The filter membrane that has adsorbed nucleic acids is washed to remove proteins and salts. Finally, low salt buffer solution (such as Buffer TE) or water can be used to wash out the nucleic acids adsorbed on the filter membrane. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

The stool samples are homogenized in the lysis solution, further lysed in a high-temperature water bath, and RNA is released into the lysis solution. Chloroform extraction removes genomic DNA and impurities, transfer the supernatant to an alcohol free binding solution, purify RNA through a column, and finally elute RNA with RNase Free Water. The purified RNA can be directly used for experiments such as PCR, Southern hybridization, and enzyme digestion.

Advantages

  • High purity - unique adsorbent for more efficient removal of inhibitors
  • High concentration - maximum extraction of total RNA from stool samples
  • Sensitive - RNA can be purified at the level of PG

Kit Contents

Contents R418502 R418503
Purification Times 50 Preps 250 Preps
HiPure RNA Mini Columns 50 250
2ml Collection Tubes
50
250
Glass Beads (0.1~0.6mm)
30 g
150 g
Buffer SPL 30 ml 140 ml
Buffer PHC
30 ml
140 ml
Buffer GRP
60 ml
250 ml
Buffer RW1
50 ml
250 ml
Buffer RW2 *
20 ml
2 x 50 ml
RNase Free Water
15 ml
30 ml


Storage and Stability

The kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions. At low temperatures, Buffer SPL may form precipitates, dissolve it by 55°C water bath. After receiving the product, Buffer PHC should be stored at 2-8°C.

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