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HiPure Blood RNA Mini Kit

R416102 HiPure Blood RNA Mini Kit 50 preps $149.00
R416103 HiPure Blood RNA Mini Kit 250 preps $646.00


The Kit provides fast purification of high-quality RNA from whole blood, cells and tissues using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol / chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. The purified RNA can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection and in vitro translation.



Features Specifications
Main Functions Isolation total RNA from 1-1.5ml whole blood
Applications qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridization and SNP detection
Purification method Mini spin column
Purification technology Silica technology, acidphenol / guanidine extraction technology (MagZol pretreatment technology)
Process method Manual (centrifugation or vacuum)
Sample type Fresh or frozen blood, mammalian blood
Sample amount 1.5 ml whole blood
Yield 2-100μg
Elution volume 20μl
Liquid carrying volume per column 800µl
Binding yield of column 100μg


The Kit simplifies isolation of RNA from blood with a fast spin-column procedure. Red blood cells are selectively lysed and white cells collected by centrifugation. White cells are then lysed using highly denaturing conditions which immediately inactivate RNases. After homogenization using the DNA spin column, the sample is applied to the RNA column. Total RNA binds to the membrane and contaminants are washed away, leaving pure RNA to be eluted in 30–100µl RNase-free water (provided with the kit) for direct use in any downstream application.


  • High quality - high purity total RNA can be directly used in various sensitive downstream applications
  • Fast - isolation of several samples can be completed in 20 minutes by using column purification method
  • Sensitive - RNA can be purified at the level of PG

Kit Contents

Contents R416102 R416103
Purification Times 50 Preps 250 Preps
HiPure DNA Mini Columns
HiPure RNA Mini Columns I 50 250
2ml Collection Tubes
10 x Buffer RBC
50 ml
3 x 100 ml
RTL Lysis Buffer 50 ml 250 ml
Buffer RW1
50 ml
250 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml

Storage and Stability

HiPure Blood RNA Mini Kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form in the RTL Lysis Buffer. Dissolve by warming buffer to 37°C.

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