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HiPure HP Plant RNA Mini Kit

R4165
CAT NO PRODUCT NAME SIZE PRICE
R416502 HiPure HP Plant RNA Mini Kit 50 preps $186.00
R416503 HiPure HP Plant RNA Mini Kit 250 preps $777.00

Introduction

HiPure HP Plant RNA Mini Kit combines guanidine isothiocyanate lysing and silica gel membrane purification technology to simplify total RNA extraction. Ultracentrifugation in CsCl purification and LiCl / ethanol precipitation are not required. The kit uses DNase digestion to completely remove DNA. It is suitable for extracting up to 100μg of total RNA (including miRNA) from plant samples less than 200mg. Several samples can be extracted within 40 minutes. The purified RNA can be directly used for RT-PCR, fluorescent quantitative RT-PCR, Northern hybridization, second generation sequencing, etc.

Details


Specifications

Features Specifications
Main Functions Isolation total RNA (include miRNA)  from <200mg difficult-to-extract plant samples (use low toxicity chloroform substitutes)
Applications RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Hard-to-extraction plant samples such as fruit and seed, grape leaves, tea
Sample amount
200 mg
Elution volume 30μl
Time per run 1-24 samples within 30 minutes
Liquid carrying volume per column 800µl
Binding yield of column 100µg


Principle

This kit uses glass fiber filter membrane purification technique, and only requires simple combination-washing-elution steps. The sample is lysed and homogenized in the solution containing guanidine salt, ethanol is added to provide appropriate binding conditions, and transferred to the purification column for centrifugation. Up to 100µg of RNA can be selectively bound to the membrane, pollutants are efficiently washed off after three times of washing, and finally the purified RNA is eluted by RNase Free Water.


Advantages

  • Completely remove DNA by using of DNase
  • High quality - one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
  • Fast - several samples can be extracted in 40 minutes by column method
  • Sensitive - RNA can be recovered at the level of PG
  • Broad spectrum - various types of plant samples can be processed by diversity of operating procedures


Kit Contents

Contents R416502 D416503
Purification Times 50 Preps 250 Preps
HiPure RNA Mini Columns 50 250
2ml Collection Tubes 100 250
DNase I
600 μl
5 x 600 μl
DNase Buffer
6 ml
30 ml
Buffer PAL
60 ml
270 ml
Buffer GXP2*
20 ml
100 ml
Buffer BDP
60 ml
270 ml
Buffer RW1 50 ml 250 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml


Storage and Stability

DNase I should be stored at -20-8°C upon arrival. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Purchase Guide

1. When dealing with woody or uncommon samples, R4150 is recommended first. R4150 contains two polysaccharide/polyphenol lysis buffer, which is the most universal product.

2. R4151 is recommended for handling common economic crop samples for the first time. Strong lysis solution can be used to process easy-extraction samples. The amount of corn or rice leaves samples can reach up to 300mg.

3. R4165 adopts CTAB/chloroform method, which can also handle a large number of difficult-to-extraction plants, but requires contact with chloroform substitutes, which is less safe than other kits. This kit uses DNase Ⅰ to remove DNA, which is also a good choice for extracting polysaccharide/polyphenol-rich plant samples.

4. R4014 is recommended for fruit/starch plant samples, which uses improved trizol pre-treatment, single column operation and is more economical.


Select the right purification kit to get impactful results

Ⅰ. Purchase guide of Magen kits based on sample

Ⅱ. Cross purchase guide for Magen kits vs Other brands

For any customized product or OEM service, please contact us.

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