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Your present location:Home/Products/DNA&RNA Purification/RNA/Tissue/Cell RNA/MagPure FFPE RNA Kit
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MagPure FFPE RNA Kit

IVD3022
CAT NO PRODUCT NAME SIZE PRICE
IVD3022 MagPure FFPE RNA Kit 200 preps $994.00

Introduction

This kit is suitable for high-throughput extraction of total RNA from paraffin embedded tissue samples, including miRNA. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 120 minutes. The obtained RNA can be directly used in RT-PCR. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 holeo scillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.

Details


Specifications

Features Specifications
Main Functions Isolation total RNA from FFPE tissue
Applications RT-PCR, cDNA synthesis, second generation sequencing
Products RNA, miRNA
Purification method Polydisperse magnetic beads
Purification technology Magnetic beads technology
Process method Manual or automatic
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Sample type FFPE slice, FFPE embedded tissue
Sample amount
6 slices


Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.


Advantages

  • Fast - several samples can be extracted in 120 minutes by column method
  • Safe - no phenol chloroform extraction required


Kit Contents

Contents IVD3022
Purification Times 200 Preps
MagBind Particles
4.5 ml
Proteinase K
100 mg
Protease Dissolve Buffer
6 ml
DNase I
4 x 600 µl
DNase Buffer
30 ml
Buffer DPS
200 ml
Buffer FRL
40 ml
Buffer AL 40 ml
Buffer MW1*
110 ml
Buffer MW2*
2 x 50 ml
Nuclease Free Water
30 ml


Storage and Stability

Proteinase K and MagBind Particles should be stored at 2–8°C upon arrival. DNase I should bestored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K and MagBind Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.

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