Introduction

The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.

Details

Specifications

Features	Specifications
Main Functions	Isolation up to 20µg plasmid DNA from 1-3ml bacterial culture
Applications	Enzyme digestion, sequencing, PCR and labeling, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Conventional plasmid, plasmid≤30KB
Sample amount	1-3ml
Elution volume	≥50μl

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
High yield - up to 20μg plasmid can be binded in one column

Kit Contents

Contents	P181102	P181103	P181104
Purification Times	100 Preps	500 Preps	5000 Preps
RNase A	10 mg	50 mg	2 x 250 mg
Buffer P1	30 ml	150 ml	2 x 750 ml
Buffer P2	30 ml	150 ml	2 x 750 ml
Buffer N3	30 ml	150 ml	2 x 750 ml
Buffer PW1	35 ml	180 ml	2 x 900 ml
MagPure Particle NB*	2.2 ml	11 ml	2 x 60 ml

Storage and Stability

RNase A and MagPure Particle NB should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2-8°C.

Experiment Data

Articles

【Nature Catalysis】Efficient exploration of terpenoid biosynthetic gene clusters in filamentous fungi

Download

P1811 MagPure Plasmid Mini Kit 2022.pdf Preview Down

CAT NO	PRODUCT NAME	SIZE	PRICE
P181102	MagPure Plasmid Mini Kit	100 preps	$59.00
P181103	MagPure Plasmid Mini Kit	500 preps	$239.00
P181104	MagPure Plasmid Mini Kit	5000 preps	$2,152.00

MagPure Plasmid Mini Kit

Introduction

Details

Principle

Articles

Download

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