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HiPure Fruit RNA Kit

R4014
CAT NO PRODUCT NAME SIZE PRICE
R401402 HiPure Fruit RNA Kit 50 preps $149.00
R401403 HiPure Fruit RNA Kit 250 preps $622.00

Introduction

The HiPure Fruit RNA Mini Kit provides fast purification of high-quality RNA from fruits, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100µg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA(mRNA) purification, nuclease protection and in vitro translation.

Details


Specifications

Features Specifications
Main Functions Isolation total RNA from <150 mg fruit or seed using chloroform and Plantzol reagent
Applications RT-PCR, qPCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation translation
Purification method Mini spin column
Purification technology Silica technology, DNA filtration technology
Process method Manual (centrifugation or vacuum)
Sample type Simple plant, petal, peel, seed
Sample amount
50-150mg
Elution volume 300μl
Time per run ≤15 minutes(1-24 samples
Liquid carrying volume per column 800µl
Binding yield of column 100µg


Principle

The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 15 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 300µl water using the Kit.


Advantages

  • Efficient DNA removal - one step RNA extraction can effectively remove genomic DNA
  • High quality - one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
  • Fast - several samples can be extracted in 15 minutes by column method
  • Wide range - samples including animals, plants, bacteria, cells, etc
  • High output - large sample amount, and the yield can be up to 200μg


Kit Contents

Contents R401402 D401403
Purification Times 50 Preps 250 Preps
HiPure RNA Mini Columns 50 250
2ml Collection Tubes 50 250
PlantZol Reagent
60 ml
200 ml
Buffer GXP
30 ml
150 ml
Buffer RW1 50 ml 200 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml


Storage and Stability

The Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.

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