| CAT NO | PRODUCT NAME | SIZE | PRICE |
| R415102 | HiPure Plant RNA Kit | 50 preps | $149.00 |
| R415103 | HiPure Plant RNA Kit | 250 preps | $622.00 |
The HiPure Plant RNA Mini Kit (R4151) is designed for routine RNA extraction from plant tissues with low levels of polysaccharides and polyphenols.
The workflow uses guanidine-based lysis combined with dual-column purification for RNA isolation and genomic DNA removal.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from <150 mg simple plant sample without chloroform |
| Applications | RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation |
| Purification method | Mini spin column |
| Purification technology | Silica technology, DNA filtration technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Economic crops |
|
Sample amount |
≤150 mg |
| Elution volume | ≥30μl |
| Time per run | ≤25 minutes |
| Liquid carrying volume per column |
800µl |
| Binding yield of column | 100µg |
The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanolis added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.
Technical Notes
Kit Contents
| Contents | R415102 | D415103 |
| Purification Times | 50 Preps |
250 Preps |
|
HiPure DNA Mini Column II |
50 |
250 |
| HiPure RNA Mini Columns | 50 |
250 |
| 2ml Collection Tubes | 100 |
2 x 250 |
|
Buffer RLC |
50 ml |
200 ml |
|
Buffer PRC1 |
50 ml |
200 ml |
| Buffer RW1 | 50 ml |
200 ml |
|
Buffer RW2* |
20 ml |
2 x 50 ml |
|
RNase Free Water |
10 ml |
30 ml |
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
HiPure Plant RNA Mini Kit (R4151) is suitable for routine plant samples with low polysaccharide and polyphenol content.
For unknown or variable samples, HiPure Plant RNA Plus Kit (R4150) is recommended as a more broadly compatible system.
For difficult plant samples, CTAB-based workflows such as HiPure HP Plant RNA Mini Kit (R4165) may provide improved performance.
For a complete selection guide:
D6381
D3141F
D3124
R4150
D6359D
R6672C
IVD4179
R4165
IVD3102
D3018
IVD5412
IVD4175
R6611
R4310
IVD3020
R4111
R6327
D6364
D3142
D3146
D6351
D3187
D3126
IVD5435
IVD3182
D6311
D3111
