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HiPure Universal DNA Kit

D301802 HiPure Universal DNA Kit 50 preps $149.00
D301803 HiPure Universal DNA Kit 250 preps $622.00


This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.



Features Specifications
Main Functions Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots
Applications PCR, qPCR, southern bolt and virus detection, etc
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Tissue, cell, blood, saliva, swab, blood spot,semen and other clinical samples
Sample amount Solid tissue: 1-10mg; Anticoagulant blood: 200μl
Elution volume ≥20μl
Time per run 30 - 60 minutes
Liquid carrying volume per column 800µl
Binding yield of column 100µg


This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).


  • High quality DNA - meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
  • Fast - without separation of leukocytes, organic extraction or ethanol precipitation
  • Simple - all nucleic acids can be obtained by direct digestion
  • Wide applicability- handle a variety of liquid samples

Kit Contents

Contents D301802 D301803
Purification Times 50 250
HiPure DNA Mini Columns I 50 250
2ml Collection Tubes 100 500
Buffer ATL 30 ml 150 ml
Buffer AL 30 ml 150 ml
Buffer GW1 22 ml 88 ml
Buffer GW2 12 ml 50 ml
Proteinase K 24 mg 120 mg
Protease Dissolve Buffer 1.8 ml 10 ml
Buffer AE 15 ml 60 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data


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