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MagPure Blood DNA Kit D6311

CAT NO PRODUCT NAME SIZE PRICE
D631101 MagPure Blood DNA Kit 48 preps $99.00
D631102 MagPure Blood DNA Kit 96 preps $174.00
D631103 MagPure Blood DNA Kit 480 preps $746.00

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.

Details


Specifications

Features Specifications
Main Functions Isolation total DNA from 200μl whole blood
Applications PCR, southern bolt and virus detection, etc
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
Sample amount 200μl
Elution volume ≥50μl
Time per run ≤60 minutes


Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

  • High binding force - suitable for handling DNA rich samples, such as whole blood, buffy coat, concentrated blood, etc.
  • Fast - polydisperse magnetic beads, fast magnetic response and short extraction time
  • High purity - the obtained DNA can be directly used for second-generation sequencing, PCR based detection, gene bank, etc.
  • Automatic - saving time and labor and safer


Kit Contents

Contents D631101 D631102 D631103
Purification Times 48 96 480
MagPure Particles 1.2 ml 2.5 ml 11 ml
Proteinase K 24 mg 50 mg 220 mg
Protease Dissolve Buffer 1.8 ml 5 ml 15 ml
Buffer AL 15 ml 30 ml 120 ml
Buffer BD 5 ml 10 ml 50 ml
Buffer GW1 22 ml 53 ml 220 ml
Elution Buffer 15 ml 30 ml 100 ml

Storage and Stability

Proteinase K, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

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