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Your present location:Home/Products/DNA&RNA Purification/DNA/RNA Coisolation/DNA/RNA Coisolation/MagPure FFPE DNA/RNA Kit
  • IVD3026.png

MagPure FFPE DNA/RNA Kit

IVD3026
CAT NO PRODUCT NAME SIZE PRICE
IVD3026 MagPure FFPE DNA/RNA Kit 200 preps $1,492.00

Introduction

The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and Pyrosequencing.

Details


Specifications

Features Specifications
Main FunctionsC Co-isolation total RNA and DNA from FFPE tissue
Applications RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
Purification method Polydisperse magnetic beads
Purification technology Magnetic beads technology
Process method Manual or automatic
Adaptive instrument
Nucleic acid extractor and pipetting workstation
Sample type FFPE slice, FFPE puncture sample, embedded tissue
Sample amount No more than six 10µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area.


Principle

FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by RNase Free Water.

Advantages

  • Post digestion sorting - higher DNA and RNA yields
  • Economy – the price is much lower than imported reagents

Kit Contents

Contents IVD3026
Purification Times 200 Preps
MagBind Particles 9.0 ml
Proteinase K
180 mg
Protease Dissolve Buffer 10 ml
Buffer DPS
150 ml
Buffer FRL 40 ml
Buffer ATL 40 ml
Buffer AL
80 ml
Buffer BXW1*
110 ml
RNase Free Water 30 ml


Storage and Stability

Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

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