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MagPure FFPE DNA Kit (High Pure) D6323D

CAT NO PRODUCT NAME SIZE PRICE
D632301D MagPure FFPE DNA Kit (High Pure) 48 preps $149.00
D632302D MagPure FFPE DNA Kit (High Pure) 96 preps $288.00

Introduction

This product is suitable for rapid extraction of DNA from FFPE sample. This kit uses two combination methods. High-salt Bind is conducive to remove pigments or polysaccharides from complex FFPE samples, so as to improve the purity of nucleic acid and avoid blocking aligent 2100. Alcohol mediated adsorption is conducive to improve the nucleic acid yield of high-yield samples.

Details


Specifications

Features Specifications
Main Functions Isolation high pure total DNA from FFPE using high bind beads
Applications PCR and viral DNA detection, etc.
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Paraffin embedded tissue samples
Sample amount 1-6 slices of 10-20μm
Elution volume ≥30μl
Time per run 60 minutes


Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

  • High yield - most optimal process, recovery up to 90%
  • Economy - less than 50% of the price of Qiagen and other imported products
  • High purity - OD 260/280 : 1.7-1.9, OD 260/230 : 1.5-2.0


Kit Contents

Contents D632301D D632302D
Purification Times 48 Preps 96 Preps
MagPure Particles N 1.1 ml 2.5 ml
RNase A 10 mg 20 mg
Proteinase K 24 mg 48 mg
Protease Dissolve Buffer 3 ml 6 ml
Buffer DPS 60 ml 100 ml
Buffer ATL
15 ml 30 ml
Buffer BST1
30 ml 60 ml
Buffer BW1
13 ml 44 ml
Elution Buffer
15 ml 30 ml


Storage and Stability

Proteinase K, RNase A and MagPure Particles N should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

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