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HiPure Tissue&Blood DNA Midi Kit

D311302 HiPure Tissue&Blood DNA Midi Kit 20 preps $224.00
D311303 HiPure Tissue&Blood DNA Midi Kit 100 preps $995.00


This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern Blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue and culture cells.



Features Specifications
Main Functions Isolation total DNA from 2ml blood and 200mg tissue using Midi column
Applications PCR, southern bolt and virus detection, etc
Purification method Midi spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples
Sample amount 0.2-2 ml
Elution volume ≥300μl
Time per run ≤80 minutes
Liquid carrying volume per column 4ml
Binding yield of column 1mg


This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).


  • High quality DNA - meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
  • Fast - without separation of leukocytes, organic extraction or ethanol precipitation
  • Simple - all nucleic acids can be obtained by direct digestion
  • Wide applicability - handle a variety of liquid samples

Kit Contents

Contents D311302 D311303
Purification Times 20 100
HiPure gDNA Midi Columns 20 100
15ml Collection Tubes 40 200
Buffer ATL 50 ml 250 ml
Buffer AL
50 ml 250 ml
Buffer GW1* 22 ml 110 ml
Buffer GW2* 12 ml 50 ml
RNase A 20 mg 90 mg
Proteinase K 100 mg 440 mg
Protease Dissolve Buffer 10 ml 30 ml
Buffer AE 20 ml 120 ml

Storage and Stability

Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

Purchase Guide


Sample amount

Leukocyte protocol*

Colum type

Elutio volume

Average yield

Time per run

HiPure Blood DNA Mini Kit D3111 10-200μl 1ml 2ml column ≥20μl 5-9μg/200μl ≤30 minutes
HiPure Tissue&Blood DNA Midi Kit D3113 0.2-2ml 10ml 1.5ml column ≥300μl 20-40μg/1m ≤80 minutes
HiPure Tissue&Blood DNA Maxi Kit D3115 3 -10ml 10ml 15ml column ≥700μl 20-40μg/1m ≤90 minutes
HiPure Tissue&Blood DNA 96 Kit D3117 1-200μl 1ml 96 well plate

*Note:Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate, and white blood cells were obtained by centrifugation before extraction

Select the right purification kit to get impactful results

Ⅰ. Purchase guide of Magen kits based on sample

Ⅱ. Cross purchase guide for Magen kits vs Other brands

For any customized product or OEM service, please contact us.

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