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HiPure Tissue DNA Kit D3121

CAT NO PRODUCT NAME SIZE PRICE
D312102 HiPure Tissue DNA Kit 50 preps $124.00
D312103 HiPure Tissue DNA Kit 250 preps $497.00

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue and culture cells.

Details


Specifications

Features Specifications
Main Functions Isolation total DNA from 30 mg tissue, culture cells, FTA card
Applications PCR, southern bolt and virus detection, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Animal tissue or cultured cells
Sample amount Animal tissue : 1-30mg, Cultured cells : <5 x 106
Elution volume ≥30μl
Time per run 30 - 60 minutes
Liquid carrying volume per column 800μl
Binding yield of column 100μg


Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while proteinis not adsorbed and is removed with filtration. After washing proteins andother impurities, Nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).

Advantages

  • Good repeatability - suitable for extracting high-yield DNA from different types of tissue samples
  • High purity - can be used for downstream applications such as multiplex and quantitative PCR
  • Fast - simplified process, extracting several samples in 20 minutes (after digestion)
  • Safety - no phenol orchloroform extraction, no alcohol precipitation


Kit Contents

Contents D312102 D312103
Purification Times 50 Preps 250 Preps
Buffer ATL 15 ml 65 ml
Buffer DL 15 ml 65 ml
Buffer GW1 22 ml 110 ml
Buffer GW2 12 ml 50 ml
RNase A 10 mg 50 mg
Proteinase K 24 mg 120 mg
Protease Dissolve Buffer
5 ml 15 ml
Buffer AE
15 ml
60 ml
HiPure gDNA Mini Columns
50 2 x 125
2 ml Collection Tubes
100 5 x 100


Storage and Stability

RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Purchase Guide

Name CAT NO

Tissue amount

Cell number

Column type

Elution volume

Yield (liver)

Yield (muscle)

HiPure Tissue DNA Kit D3121 1 - 30mg 5 x 106 1.5ml column 30 - 100μl 35μg/10mg g/10mg
HiPureDNA Micro Kit D3125 <2mg 1 x 105 1.5ml column 15 - 50μl 3.6μg/1mg 0.89μg/1mg

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