Introduction

The HiPure Minipreps system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

Details

Specifications

Features	Specifications
Main Functions	Isolation up to 30μg endotoxin-free plasmid DNA from 1-5ml bacterial culture using 96-well bind plate
Applications	Cell transfection, animal injection, etc.
Purification method	96 well plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid, plasmid less than 30KB
Sample amount	1-5ml（x96）
Yield	30μg
Elution volume	≥75μl
Time per run	≤60 minutes
Liquid carrying volume per column	800μl
Binding yield of column	70μg

Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast - silica gel column purification is much faster than ion exchange
Ultra low endotoxin - content of endotoxin is less than 0.05 EU/μg, which can be directly used for cell transfection and animal injection, etc.

Kit Contents

Contents	P115701	P115702	P115703
Purification Times	1 x 96 Preps	4 x 96 Preps	20 x 96 Preps
RNase A	10 mg	20 mg	100 mg
Buffer P1	30 ml	120 ml	550 ml
Buffer P2	30 ml	120 ml	550 ml
Buffer LEN3	15 ml	60 ml	300 ml
Buffer LN4	80 ml	270 ml	2 x 700 ml
Buffer LN5	40 ml	220 ml	1100 ml
Buffer PW1	40 ml	220 ml	1100 ml
Buffer PW2	25 ml	100 ml	2 x 200 ml
Elution Buffer	30 ml	60 ml	250 ml
Lysate Clear Plate	1	4	20
HiPure DNA Plate	1	4	20
1.6ml Collection Plate	2	8	40
0.5ml Collection Plate	1	4	20

Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.

Purchase Guide

Name	CAT NO	Sample amount	Column type	Elution volume	Yield	Time per run	Centrifuge requirements
HiPure Plasmid EF Mini Kit	P1154	10-15ml	1.5ml column	≥75μl	10–70µg/15ml	≤40 minutes	small centrifuge
HiPure Plasmid EF 96 Kit	P1157	1-5ml	96 well plate	≥75μl	30µg/1ml	≤60 minutes	96 well plate centrifuge
HiPure Plasmid EF Midi Kit	P1231	30-50ml	15ml column	≥0.4ml	10-500μg/50ml	≤60 minutes	15ml centrifuge
HiPure Plasmid EF Maxi Kit	P1156	100-200ml	50ml column	≥0.8ml	100–1500µg/200ml	≤60 minutes	50ml centrifuge
HiPure Plasmid EF Mega Kit	P1116	500ml	50ml column	≥0.8ml	10mg/500ml	≤70 minutes	50ml centrifuge

Articles

Download

P1157 HiPure Plasmid EF 96 Kit.pdf Preview Down

CAT NO	PRODUCT NAME	SIZE	PRICE
P115701	HiPure Plasmid EF 96 Kit	1 x 96 preps	$224.00
P115702	HiPure Plasmid EF 96 Kit	4 x 96 preps	$646.00
P115703	HiPure Plasmid EF 96 Kit	20 x 96 preps	$2735.00

HiPure Plasmid EF 96 Kit

Introduction

Details

Principle

Purchase Guide

Articles

Download

Related Products

HiPure Plasmid Mini Kit II

HiPure Plasmid EF Mini Kit

HiPure Plasmid EF Midi Kit

Popular products

HiPure Universal DNA Kit

MagPure Viral DNA/RNA Kit

HiPure Viral DNA/RNA Kit

MagPure Universal RNA Kit

HiPure Total RNA Kit

MagPure Stool DNA Kit

HiPure Stool DNA Kit

HiPure Bacterial DNA Kit

MagPure Plant DNA Kit

HiPure HP Plant DNA Kit

HiPure DNA Micro Kit

HiPure FFPE DNA Kit

MagPure Circulating DNA Mini Kit

HiPure Circulating DNA Midi Kit C (Vaccum Protocol)

MagPure Universal DNA Kit

HiPure Blood DNA Mini Kit