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HiPure Plasmid EF Maxi Kit

P1156
CAT NO PRODUCT NAME SIZE PRICE
P115602 HiPure Plasmid EF Maxi Kit 10 preps $164.00
P115603 HiPure Plasmid EF Maxi Kit 50 preps $746.00

Introduction

The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen's innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.

Details


Specifications

Features Specifications
Main Functions Isolation up to 1.5mg endotoxin-free plasmid DNA from 200ml bacterial culture
Applications Cell transfection, animal injection, etc.
Purification method Maxi spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type
Conventional plasmid, large plasmid
Sample amount

High copy plasmid: 100-150ml culture medium

Low copy plasmid: 150-200ml culture medium

Yield 100-1500μg
Elution volume ≥0.8ml
Time per run 60 minutes
Liquid carrying volume per column 20ml
Binding yield of column 1mg


Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).


Advantages

  • High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
  • Fast - silica gel column purification is much faster than ion exchange
  • High yield - up to 1.5mg plasmid can be binded in one column
  • Ultra low endotoxin - content of endotoxin is less than 0.1 EU/μg, which can be directly used for cell transfection and animal injection, etc.


Kit Contents

Contents P115602 P115603
Purification Times 10 Preps 50 Preps
RNase A 30 mg 150 mg
Buffer P1 100 ml 500 ml
Buffer P2
100 ml
500 ml
Buffer LN3
50 ml
250 ml
Buffer PW1
33 ml
180 ml
Buffer PW2 20 ml 100 ml
Elution Buffer 15 ml 120 ml
Buffer CL 33 ml 180 ml
HiPure DNA Maxi Columns C
10
50
Lysate Clear Midi Syringe
10
50
50 ml Collection Tubes C
20
100


Storage and Stability

The kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2–8°C.

Purchase Guide

Name CAT NO

Sample amount

Column type

Elution volume

Yield

Time per run

Centrifuge requirements
HiPure Plasmid EF Mini Kit P1154 10-15ml 1.5ml column 75μl

10–70µg/15ml

≤40 minutes small centrifuge
HiPure Plasmid EF 96 Kit P1157 1-5ml 96 well plate 75μl

30µg/1ml

≤60 minutes 96 well plate centrifuge
HiPure Plasmid EF Midi Kit P1231 30-50ml 15ml column ≥0.4ml

10-500μg/50ml

≤60 minutes 15ml centrifuge
HiPure Plasmid EF Maxi Kit P1156 100-200ml 50ml column ≥0.8ml

100–1500µg/200ml

60 minutes
50ml centrifuge
HiPure Plasmid EF Mega Kit
P1116
500ml
50ml column
≥0.8ml

10mg/500ml

70 minutes
50ml centrifuge

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