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Your present location:Home/Products/DNA&RNA Purification/DNA/Tissue DNA/HiPure Tissue&Blood DNA Maxi Kit
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HiPure Tissue&Blood DNA Maxi Kit

D3115
CAT NO PRODUCT NAME SIZE PRICE
D311502 HiPure Tissue&Blood DNA Maxi Kit 10 preps $174.00
D311503 HiPure Tissue&Blood DNA Maxi Kit 50 preps $622.00

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from whole blood, tissue and culture cells.

Details


Specifications

Features Specifications
Main Functions Isolation total DNA from 10ml blood and 1g tissue using Maxi column
Applications PCR, southern bolt and virus detection, etc
Purification method Maxi spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples
Sample amount 3-10ml
Elution volume ≥700μl
Time per run ≤90 minutes
Liquid carrying volume per column 4ml
Binding yield of column 5mg


Principle

This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

  • High quality DNA - meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
  • Fast - without separation of leukocytes, organic extraction or ethanol precipitation
  • Simple - all nucleic acids can be obtained by direct digestion
  • Pertinence - specially designed for isolating DNA from 3-10ml blood and related body fluids
  • Wide applicability - handle a variety of liquid samples
  • Economy - excellent cost effectiveness performance

Kit Contents

Contents D311502 D311503
Purification Times 10 50
HiPure gDNA Maxi Columns 10 50
50ml Collection Tubes 20 100
Buffer ATL 120 ml 550 ml
Buffer AL
120 ml 2 x 300 ml
Buffer GW1* 53 ml 220 ml
Buffer GW2* 25 ml 110 ml
RNase A 40 mg 180 ml
Proteinase K 120 mg 540 mg
Protease Dissolve Buffer 12 ml 50 ml
Buffer AE 30 ml 120 ml

Storage and Stability

Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

Name CAT NO

Sample amount

Leukocyte protocol*

Colum type

Elutio volume

Average yield

Time per run

HiPure Blood DNA Mini Kit D3111 10-200μl 1ml 2ml column ≥20μl 5-9μg/200μl ≤30 minutes
HiPure Tissue&Blood DNA Midi Kit D3113 0.2-2ml 10ml 1.5ml column ≥300μl 20-40μg/1m ≤80 minutes
HiPure Tissue&Blood DNA Maxi Kit D3115 3 -10ml 10ml 15ml column ≥700μl 20-40μg/1m ≤90 minutes
HiPure Tissue&Blood DNA 96 Kit D3117 1-200μl 1ml 96 well plate
3-8μg/200μl

*Note:Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate, and white blood cells were obtained by centrifugation before extraction

Select the right purification kit to get impactful results

Ⅰ. Purchase guide of Magen kits based on sample

Ⅱ. Cross purchase guide for Magen kits vs Other brands

For any customized product or OEM service, please contact us.

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