| CAT NO | PRODUCT NAME | SIZE | PRICE |
| R415202 | HiPure Plant RNA Midi Kit | 20 preps | $398.00 |
| R415203 | HiPure Plant RNA Midi Kit | 100 preps | $1,740.00 |
The HiPure Plant RNA Midi Kit (R4152) extends the R4151 workflow for larger sample inputs, supporting up to gram-scale plant tissue processing.
It is designed for applications requiring higher RNA yield from routine plant samples.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from <1g simple plant sample without chloroform |
| Applications | RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation |
| Purification method | Midi spin column |
| Purification technology | Silica technology, DNA filtration technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Plant leaves, roots, stems, fruits, fungi, etc. |
|
Sample amount |
0.5-1 g |
| Elution volume | ≥400μl |
| Time per run | ≤40 minutes |
| Liquid carrying volume per column |
4ml |
| Binding yield of column | 1mg |
The Kit isolates total RNA from up to 1g plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 40 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 400µl water using the Kit.
Advantages
Kit Contents
| Contents | R415202 | D415203 |
| Purification Times | 20 Preps |
100 Preps |
|
HiPure DNA Midi Column II |
20 |
100 |
| HiPure RNA Midi Columns II | 20 |
100 |
| 15ml Collection Tubes | 40 |
200 |
|
Buffer RLC |
120 ml |
550 ml |
|
Buffer PRC1 |
120 ml |
550 ml |
|
Buffer RW1 |
70 ml |
400 ml |
|
Buffer RW2* |
20 ml |
100 ml |
|
RNase Free Water |
30 ml |
120 ml |
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
HiPure Plant RNA Midi Kit (R4152) is designed for large-scale RNA extraction from routine plant samples.
For smaller-scale extractions, HiPure Plant RNA Mini Kit (R4151) may be used.
For variable or complex plant samples, HiPure Plant RNA Plus Kit (R4150) is recommended.
For a complete selection guide:
D3124
R4150
D6359D
R6672C
IVD3102
R4111
R6327
D3126
IVD5435
IVD3182
D3111
