| CAT NO | PRODUCT NAME | SIZE | PRICE |
| R214402 | HiPure RNA Clean Up Kit | 50 preps | $120.00 |
| R214403 | HiPure RNA Clean Up Kit | 250 preps | $430.00 |
The HiPure RNA Clean Up Kit provides a method for purification and concentration of RNA from enzymatic reactions and RNA samples. The workflow removes salts and contaminants while maintaining RNA integrity.
This system is commonly used for RNA desalting, concentration and preparation for downstream applications such as RT-PCR and transcription-based assays.
Specifications
| Features | Specifications |
| Main Functions | Purification and concentration of RNA（mRNA>200nt or miRNA）from transcription products, DNase cleavage products, labeled products, and crude RNA products |
| Applications |
Sequencing, ligation, enzyme digestion, RT-PCR, labeling, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type |
Crude RNA, enzymatic reaction solution |
| Sample amount | ≤100μl |
|
Recovery |
90% |
| Elution volume | ≥15μl |
| Time per run |
≤15 minutes |
| Liquid carrying volume per column |
800μl |
| Binding yield of column | 100μg |
The HiPure system uses a simple bind-wash-elute procedure. Binding buffer is added directly to the sample or other enzymatic reaction, and the mixture is applied to the column. Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure RNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications.
Advantages
Kit Contents
| Contents | R214402 | R214403 |
| Purification Times | 50 Preps |
250 Preps |
| Buffer GXP | 30 ml | 120 ml |
|
Buffer RW2 |
20 ml |
2 x 50 ml |
| RNase-Free Water |
20 ml |
60 ml |
| HiPure RNA Mini Columns I | 50 |
250 |
|
2 ml Collection Tubes |
50 |
250 |
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
For guidance on selecting the most appropriate DNA extraction system based on sample type, input volume and workflow requirements:
D6381
D3141F
D3124
R4150
D6359D
R6672C
IVD4179
R4165
IVD3102
D3018
IVD5412
IVD4175
R6611
R4310
IVD3020
R4111
R6327
D6364
D3142
D3146
D6351
D3187
D3126
IVD5435
IVD3182
D6311
D3111
